Employees – University of Copenhagen

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Department of Drug Design and Pharmacology > Employees

Bente Vestergaard

Bente Vestergaard

Professor with special responsibilities

I am the group leader of the BioSAXS group, dept. Drug Design and Pharmacology, University of Copenhagen. have focused on structural biology throughout my carreer. I was trained in macromolecular X-ray crystallography and have experience with cryo electron microscopy, atomic force microscopy and fiber diffraction. Since 2004 I have specialized in small angle X-ray scattering on proteins in solution and have established the first Scandinavian bioSAXS group at the University of Copenhagen. In my group, we focus on structural characterisation of complex pharmaceutically relevant protein solutions. We analyze heterogeneous protein systems, and particularly specialize in characterisation of fibrillating proteins. We have developed novel methods for structural analysis of fibrillating systems, applying advanced decomposition and multidisciplinary characterisation.

Primary fields of research

BioSAXS

Structural Analysis of Protein Fibrillation

Structural Biology

Teaching

National Teaching: course responsible for 'Advances in Medicinal Chemistry Research'. Lecturer and supervisor of practicals on 'Medicinal and Biostructural Chemistry'.

International Teaching: Co-organizer (together with my close colleague prof. Lise Arleth) of the Copenhagen BioSAS practial courses on biomacromolecular solution scattering. The courses aim at international young researchers (PhD, postdoc level) and is a collaboration with the group of EMBL group leader Dmitri Svergun and MAX-lab SAXS beamline (I911-4). I am returning teacher on the EMBL-Hamburg EMBO BioSAS courses, and (more recently) the ESRF Grenoble EMBO BioSAS course.

Selected publications

  1. Published

    A helical structural nucleus is the primary elongating unit of insulin amyloid fibrils

    Vestergaard, B., Jensen, M. G., Roessle, M., Kastrup, J. S. J., Van De Weert, M., Flink, J. M., Frøkjær, S., Gajhede, M. & Svergun, D. I. 2007 In : PLoS - Biology. 5, 5, p. 1089-1097

    Publication: Research - peer-reviewJournal article

  2. Published

    Ionotropic glutamate-like receptor d2 binds D-serine and glycine.

    Naur, P., Hansen, K. B., Kristensen, A. S., Dravid, S. M., Pickering, D. S., Olsen, L., Vestergaard, B., Egebjerg, J., Gajhede, M., Traynelis, S. F. & Kastrup, J. S. J. 2007 In : Proceedings of the National Academy of Science of the United States of America. 104, 35, p. 14116-14121

    Publication: Research - peer-reviewJournal article

  3. Published

    Small angle X-ray scattering study of calreticulin reveals conformational plasticity

    Toft, K. N., Larsen, N., Jørgensen, F. S., Højrup, P., Houen, G. & Vestergaard, B. 2008 In : Biochimica et Biophysica Acta. 1784, 9, p. 1265-1270

    Publication: Research - peer-reviewJournal article

  4. Published

    High-throughput small angle X-ray scattering from proteins in solution using a microfluidic front-end

    Toft, K. N., Vestergaard, B., Nielsen, S. S., Snakenborg, D., Jeppesen, M. G., Jacobsen, J. K., Arleth, L. & Kutter, J. P. 2008 In : Analytical Chemistry. 80, 10, p. 3648-3654 7 p.

    Publication: Research - peer-reviewJournal article

  5. Published
  6. Published

    Application of Bayesian analysis to indirect fourier transformation in small-angle scattering

    Vestergaard, B. & Hansen, S. L. 2006 In : Journal of Applied Crystallography. 39, p. 797-804 8 p.

    Publication: Research - peer-reviewJournal article

  7. Published

    Small angle X-ray studies reveal that Aspergillus niger glucoamylase has a defined extended conformation and can form dimers in solution

    Jørgensen, A. D., Nøhr, J., Kastrup, J. S., Gajhede, M., Sigurskjold, B. W., Sauer, J., Svergun, D. I., Svensson, B. & Vestergaard, B. 2008 In : Journal of Biological Chemistry. 283, 21, p. 14772-14780

    Publication: Research - peer-reviewJournal article

  8. Published

    A new small-angle X-ray scattering set-up on the crystallography beamline I711 at MAX-lab

    Knaapila, M., Svensson, C., Barauskas, J., Zackrisson, M., Nielsen, S. S., Toft, K. N., Vestergaard, B., Arleth, L., Olsson, U., Pedersen, J. S. & Cerenius, Y. 2009 In : Journal of Synchrotron Radiation. 16, 4, p. 498-504 7 p.

    Publication: Research - peer-reviewJournal article

  9. Published

    BioXTAS RAW, a software program for high-throughput automated small-angle X-ray scattering data reduction and preliminary analysis

    Nielsen, S. S., Toft, K. N., Snakenborg, D., Jeppesen, M. G., Jacobsen, J. K., Vestergaard, B., Kutter, J. P. & Arleth, L. 2009 In : Journal of Applied Crystallography. 42, 5, p. 959-964 6 p.

    Publication: Research - peer-reviewJournal article

  10. Published
  11. Published

    Methods for structural characterization of prefibrillar intermediates and amyloid fibrils

    Langkilde, A. E. & Vestergaard, B. 2009 In : FEBS Letters. 583, 16, p. 2600-2609

    Publication: Research - peer-reviewJournal article

  12. Published

    Extensive small-angle X-ray scattering studies of blood coagulation factor VIIa reveal interdomain flexibility

    Mosbæk, C. R., Nolan, D., Persson, E., Svergun, D. I., Bukrinsky, J. T. & Vestergaard, B. 2010 In : Biochemistry. 49, 45, p. 9739-9745

    Publication: Research - peer-reviewJournal article

  13. Published

    Formation mechanism of insulin fibrils and structural aspects of the insulin fibrillation process

    Jensen, M. G., Frøkjær, S. & Vestergaard, B. 2009 In : Current Protein and Peptide Science. 10, 5, p. 509-528

    Publication: Research - peer-reviewJournal article

  14. Published

    Self-association of long-acting insulin analogues studied by size exclusion chromatography coupled to multi-angle light scattering

    Jensen, M. H., Wahlund, P-O., Jacobsen, J. K., Vestergaard, B., van de Weert, M. & Havelund, S. 2011 In : Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 879, 28, p. 2945-2951

    Publication: Research - peer-reviewJournal article

  15. Bacterial polypeptide release factor RF2 is structurally distinct from eukaryotic eRF1

    Vestergaard, B., Van, L. B., Andersen, G. R., Nyborg, J. & Kjeldgaard, M. 2001 In : Molecular Cell. 8, p. 1375-1382 7 p.

    Publication: Research - peer-reviewJournal article

  16. Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains

    Guo, Q., Vestergaard, B., Costa, Degres, Wong, Grosjean, Zhu, Wong & Xue 2002 In : Journal of Biological Chemistry. 277, p. 14343-14349 6 p.

    Publication: Research - peer-reviewJournal article

  17. A cryo-electron microscopic study of ribosome-bound termination factor RF2

    Rawat, Zavialov, A., Sengupta, Valle, Grasucci, Linde, Vestergaard, B., Ehrenberg, M. & Frank, J. 2003 In : Nature. 421, p. 87-90 4 p.

    Publication: Research - peer-reviewJournal article

  18. Structure of the E. coli ribosomal termination complex with release factor 2

    Klaholz, B., Pape, T., Zavialov, A., Myasnikov, Orlova, Vestergaard, B., Ehrenberg, M. & van Heel, M. 2003 In : Nature. 421, p. 90-94 5 p.

    Publication: Research - peer-reviewJournal article

  19. Published

    Crystal structure of the kainate receptor GluR5 ligand-binding core in complex with (S)-glutamate

    Naur, P., Vestergaard, B., Skov, L., Egebjerg, J., Gajhede, M. & Kastrup, J. S. J. 2005 In : FEBS Letters. 579, p. 1154-60

    Publication: Research - peer-reviewJournal article

  20. Published

    Diverse bacterial genomes encode an operon of two genes, one of which is a unusual class-I release factor that potentially recognizes atypical mRNA signals other than normal stop codons

    Baranov, P., Vestergaard, B., Hamelryck, T. W., Gesteland, Nyborg, J. & Atking, J. 2006 In : Biology Direct. 13, p. 1-28 28 p.

    Publication: Research - peer-reviewJournal article

  21. Published

    A high-affinity, dimeric inhibitor of PSD-95 bivalently interacts with PDZ1-2 and protects against ischemic brain damage

    Bach, A., Clausen, B. H., Møller, M., Vestergaard, B., Chi, C. N., Round, A., Sørensen, P. L., Nissen, K. B., Kastrup, J. S., Gajhede, M., Jemth, P., Kristensen, A. S., Lundström, P., Lambertsen, K. L. & Strømgaard, K. . C. 28 Feb 2012 In : National Academy of Sciences. Proceedings. 109, 9, p. 3317-3322

    Publication: Research - peer-reviewJournal article

  22. Published

    Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle X-ray scattering

    Langkilde, A. E. & Vestergaard, B. 2012 Amyloid proteins: methods and protocols. Sigurdsson, E. M., Calero, M. & Gasset, M. (eds.). 2 ed. New York: Springer Science+Business Media B.V., Vol. 849, Ch. 10, p. 137-155 18 p. (Methods in Molecular Biology, Vol. 849).

    Publication: ResearchBook chapter

  23. Published

    Small angle X-ray scattering-based elucidation of the self-association mechanism of human insulin analogue lys(B29)(N(e)¿-carboxyheptadecanoyl) des(B30)

    Jensen, M. H., Wahlund, P-O., Toft, K. N., Jacobsen, J. K., Steensgaard, D. B., van de Weert, M., Havelund, S. & Vestergaard, B. 15 Jan 2013 In : Biochemistry. 52, 2, p. 282-94 13 p.

    Publication: Research - peer-reviewJournal article

  24. Published

    Large-scale polymorphism and auto-catalytic effect in insulin fibrillogenesis

    Foderà, V., van de Weert, M. & Vestergaard, B. 2010 In : Soft Matter. 6, 18, p. 4413-4419

    Publication: Research - peer-reviewJournal article

  25. Published

    The SAXS Solution Structure of RF1 Differs from Its Crystal Structure and Is Similar to Its Ribosome Bound Cryo-EM Structure

    Vestergaard, B., Sanyal, S., Roessle, M., Mora, L., Buckingham, R., Kastrup, J. S. J., Gajhede, M., Svergun, D. I. & Ehrenberg, M. 2005 In : Molecular Cell. 20, p. 929-938

    Publication: Research - peer-reviewJournal article

  26. Published

    Unlocked Concanavalin A Forms Amyloid-like Fibrils from Coagulation of Long-lived “Crinkled” Intermediates

    Vetri, V., Leone, M., Morozova-Roche, L. A., Vestergaard, B. & Foderà, V. 2013 In : PLoS ONE. 8, 7, 11 p., e68912

    Publication: Research - peer-reviewJournal article

  27. Published

    Structural Investigations of on-pathway Oligomers of α-Synuclein

    Pedersen, M. N., Horvath, I., Weise, C. F., Foderà, V., Wittung-Stafshede, P., Wolf-Watz, M., Toft, K. N. & Vestergaard, B. 31 May 2012

    Publication: ResearchPoster

  28. Published

    High concentration formulation studies of an IgG2 antibody using small angle X-ray scattering

    Mosbæk, C. R., Konarev, P., Svergun, D. I., Rischel, C. & Vestergaard, B. 2012 In : Pharmaceutical Research. 29, 8, p. 2225-2235

    Publication: Research - peer-reviewJournal article

  29. Published

    Structural model and trans-interaction of the entire ectodomain of the olfactory cell adhesion molecule

    Kulahin, N., Kristensen, O., Rasmussen, K. K., Olsen, L., Rydberg, P., Vestergaard, B., Kastrup, J. S., Berezin, V., Bock, E., Walmod, P. S. & Gajhede, M. 9 Feb 2011 In : Structure. 19, 2, p. 203-211

    Publication: Research - peer-reviewJournal article

  30. Published

    Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

    Lafleur, J., Snakenborg, D., Nielsen, S. S., Møller, M., Toft, K. N., Menzel, A., Jacobsen, J. K., Vestergaard, B., Arleth, L. & Kutter, J. P. 2011 In : Journal of Applied Crystallography. 44, p. 1090-1099 10 p.

    Publication: Research - peer-reviewJournal article

  31. Published

    Tyr702 is an important determinant of agonist binding and domain closure of the ligand-binding core of GluR2

    Frandsen, A., Pickering, D. S., Vestergaard, B., Kasper, C., Nielsen, B. B., Greenwood, J. R., Campiani, G., Fattorusso, C., Gajhede, M., Schousboe, A. & Kastrup, J. S. 2005 In : Molecular Pharmacology. 67, 3, p. 703-13 10 p.

    Publication: Research - peer-reviewJournal article

  32. Published

    Low-resolution structure of a vesicle disrupting a-synuclein oligomer that accumulates during fibrillation

    Giehm, L., Svergun, D. I., Otzen, D. E. & Vestergaard, B. 2011 In : National Academy of Sciences. Proceedings. 108, 8, p. 3246-3251

    Publication: Research - peer-reviewJournal article

  33. Published

    Time-resolved SAXS measurements facilitated by online HPLC buffer exchange

    Jensen, M. H., Toft, K. N., David, G., Havelund, S., Pérez, J. & Vestergaard, B. 2010 In : Journal of Synchrotron Radiation. 17, 6, p. 769-773

    Publication: Research - peer-reviewJournal article

  34. Published

    Gennembrud i forståelsen af insulinfibrillering

    Jensen, M. G., Vestergaard, B., Frøkjær, S., Gajhede, M., Flink, J. M., Van De Weert, M., Kastrup, J. S. J. & Svergun, D. I. 2007 In : Lægemiddelforskning. p. 10-11

    Publication: CommunicationJournal article

  35. Published

    Kimen til de farlige fibriller

    Jensen, M. G. & Vestergaard, B. 2007 In : Aktuel Naturvidenskab. 6, p. 12-15

    Publication: CommunicationJournal article

ID: 1305569