MS-ESQUIRE-A: Bruker Esquire 3000+ Ion Trap MS
This instrument is based on an Agilent 1200 HPLC connected via an ESI-interface to an ion-trap type of mass spectrometer. The ion-trap collects ions and keeps them for a short period in a circular path. By varying the radiofrequency field of the trap, ions can be further fragmented and the weight of the fragments can be determined by a scanning process.
The instrument is suitable both for peptides and small
This instrument is only for licensed users (primarily PhD-students, post-docs
Read the primer/SOP: MS-ESQUIRE-A_SOP.pdf
Booking of instrument
The instrument is running mostly as
Data retrieval
The MS-data is automatically zipped and copied to the O-drive of the SUND-network whenever new data is acquired, processed or a pdf is saved in the data folder. Hence, there is no need to manually copy the data using an USB-device. The script responsible for the data transfer is currently running twice an hour.
Access to the ILF O-drive is administered by the IT-department on SUND. Use
Browse to O:\FTP\MS\MS-ESQUIRE-A\Data
and find the appropriate data folder (date). It will contain one zip-file per dataset. Copy and unpack the P
-drive. Find the relevant pdf-files and print them from your own computer. The workstation by the instrument is currently not connected to a printer.
Data handling and archiving
Data is not deleted from the workstation. Old data (a few months) is moved to a subfolder ILF2014
ILF2015
ILF2016
OldData
-folder on the D-drive. Occasionally, the data is backed up, but it is the users own responsibility to make sure that the data is copied to a more secure location where it is backed up on a regular basis (like the P-drive).
Data copied to the O-drive is automatically deleted after 14 days. This storage is only meant for easy transfer of the data.