MS-ESQUIRE-A: Bruker Esquire 3000+ Ion Trap MS

This instrument is based on an Agilent 1200 HPLC connected via an ESI-interface to an ion-trap type of mass spectrometer. The ion-trap collects ions and keeps them for a short period in a circular path. By varying the radiofrequency field of the trap, ions can be further fragmented and the weight of the fragments can be determined by a scanning process.

The instrument is suitable both for peptides and small molecules, and can be made to do MSn-analysis (i.e., select fragments for further fragmentation), to give more information about the structure. Typically, it will give an accurate mass with one decimal.

This instrument is only for licensed users (primarily PhD-students, post-docs and permanent staff, not students). Please contact Louise Kjærulff (louisek@sund.ku.dk) for an introduction to the instrument. All costs (solvents, guard columns, columns, repairs, service) are shared among the users in proportion to the usage.

Read the primer/SOP: MS-ESQUIRE-A_SOP.pdf

Booking of instrument

The instrument is running mostly as an walk-up-instrument. However, for some type of experiments the instrument has to be reserved for a single user. The booking must be coordinated with Louise Kjærlulff (louisek@sund.ku.dk) at least a week in advance so that the regular users can be notified. There is a calendar by the instrument where the reservations can be seen.

Data retrieval

The MS-data is automatically zipped and copied to the O-drive of the SUND-network whenever new data is acquired, processed or a pdf is saved in the data folder. Hence, there is no need to manually copy the data using an USB-device. The script responsible for the data transfer is currently running twice an hour.

Access to the ILF O-drive is administered by the IT-department on SUND. Use cable or log in via https://vpn.sund.ku.dk (employed or SUND-computers) or http://remote.sund.ku.dk (students or non-SUND computers).

Browse to O:\FTP\MS\MS-ESQUIRE-A\Dataand find the appropriate data folder (date). It will contain one zip-file per dataset. Copy and unpack the zip-file to another location, possibly on your own hard drive or your P-drive. Find the relevant pdf-files and print them from your own computer. The workstation by the instrument is currently not connected to a printer.

Data handling and archiving

Data is not deleted from the workstation. Old data (a few months) is moved to a subfolder named ILF2014, ILF2015, ILF2016,.. in the folder OldData-folder on the D-drive. Occasionally, the data is backed up, but it is the users own responsibility to make sure that the data is copied to a more secure location where it is backed up on a regular basis (like the P-drive).

Data copied to the O-drive is automatically deleted after 14 days. This storage is only meant for easy transfer of the data.

MS-Esquire-A, room 30-K26

MS-Esquire-A, room 30-K26