Frederik Ravnkilde Marlet
Frederik Ravnkilde Marlet

PhD fellow

Identification of lipid changes associated with Parkinson’s Disease and alpha-synuclein pathology in dopaminergic neuronal cells.
 

My current focuses are:

1. Development of novel PD model by using human derived neuroblastoma cell line (SH-SY5Y) and investigate the lipid composition in both the healthy and diseased model. PD-like symptoms are established in the SH-SY5Y cells by GBA knockout and oxidative stress repectively.

2. Investigate the affect of lipid vesicles on a-synuclein aggregation. The vesicles are prepared from various sphingolipids differing in size and side chains bound to the sphingosine backbone. These lipids are proven to be the most abundant lipids in the brain and the levels of these indivdual lipids are affected in PD.

3. Identification of fibril characteristics by electron microscopy. Fibrils formed by aggregation of a-synuclein in presence of lipid vesicles prepared from different sphingolipids, are used. 
 

Methods:

- Protein expression and purification
- Tht aggregation experiment
- Protein-lipid binding assay (Circular dichroism)
- Preparation of lipid vesicles
- Fibril formation
- Electron microscopy
- Cell culturing
- Lipid extraction
- Lipidomics

ID: 124862180