A novel analytical method for in vivo phosphate tracking

Research output: Contribution to journalJournal article

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A novel analytical method for in vivo phosphate tracking. / Gu, Hong; Lalonde, Sylvie; Okumoto, Sakiko; Looger, Loren L.; Scharff-Poulsen, Anne Marie; Grossman, Arthur R.; Kossmann, Jens; Jakobsen, Iver; Frommer, Wolf B.

In: FEBS Letters, Vol. 580, No. 25, 2006, p. 5885-5893.

Research output: Contribution to journalJournal article

Harvard

Gu, H, Lalonde, S, Okumoto, S, Looger, LL, Scharff-Poulsen, AM, Grossman, AR, Kossmann, J, Jakobsen, I & Frommer, WB 2006, 'A novel analytical method for in vivo phosphate tracking', FEBS Letters, vol. 580, no. 25, pp. 5885-5893. https://doi.org/10.1016/j.febslet.2006.09.048

APA

Gu, H., Lalonde, S., Okumoto, S., Looger, L. L., Scharff-Poulsen, A. M., Grossman, A. R., ... Frommer, W. B. (2006). A novel analytical method for in vivo phosphate tracking. FEBS Letters, 580(25), 5885-5893. https://doi.org/10.1016/j.febslet.2006.09.048

Vancouver

Gu H, Lalonde S, Okumoto S, Looger LL, Scharff-Poulsen AM, Grossman AR et al. A novel analytical method for in vivo phosphate tracking. FEBS Letters. 2006;580(25):5885-5893. https://doi.org/10.1016/j.febslet.2006.09.048

Author

Gu, Hong ; Lalonde, Sylvie ; Okumoto, Sakiko ; Looger, Loren L. ; Scharff-Poulsen, Anne Marie ; Grossman, Arthur R. ; Kossmann, Jens ; Jakobsen, Iver ; Frommer, Wolf B. / A novel analytical method for in vivo phosphate tracking. In: FEBS Letters. 2006 ; Vol. 580, No. 25. pp. 5885-5893.

Bibtex

@article{5e20a260a1c111ddb6ae000ea68e967b,
title = "A novel analytical method for in vivo phosphate tracking",
abstract = "Genetically-encoded fluorescence resonance energy transfer (FRET) sensors for phosphate (Pi) (FLIPPi) were engineered by fusing a predicted Synechococcus phosphate-binding protein (PiBP) to eCFP and Venus. Purified fluorescent indicator protein for inorganic phosphate (FLIPPi), in which the fluorophores are attached to the same PiBP lobe, shows Pi-dependent increases in FRET efficiency. FLIPPi affinity mutants cover Pi changes over eight orders of magnitude. COS-7 cells co-expressing a low-affinity FLIPPi and a Na+/Pi co-transporter exhibited FRET changes when perfused with 100µM Pi, demonstrating concentrative Pi uptake by PiT2. FLIPPi sensors are suitable for real-time monitoring of Pi metabolism in living cells, providing a new tool for fluxomics, analysis of pathophysiology or changes of Pi during cell migration.",
keywords = "Former LIFE faculty, Fluorescence energy transfer, Phosphate starvation, Biosensor, Synechococcus",
author = "Hong Gu and Sylvie Lalonde and Sakiko Okumoto and Looger, {Loren L.} and Scharff-Poulsen, {Anne Marie} and Grossman, {Arthur R.} and Jens Kossmann and Iver Jakobsen and Frommer, {Wolf B.}",
note = "FLIPPi, fluorescent indicator protein for inorganic phosphate, FRET, fluorescence resonance energy transfer, FP, fluorescent protein",
year = "2006",
doi = "10.1016/j.febslet.2006.09.048",
language = "English",
volume = "580",
pages = "5885--5893",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "25",

}

RIS

TY - JOUR

T1 - A novel analytical method for in vivo phosphate tracking

AU - Gu, Hong

AU - Lalonde, Sylvie

AU - Okumoto, Sakiko

AU - Looger, Loren L.

AU - Scharff-Poulsen, Anne Marie

AU - Grossman, Arthur R.

AU - Kossmann, Jens

AU - Jakobsen, Iver

AU - Frommer, Wolf B.

N1 - FLIPPi, fluorescent indicator protein for inorganic phosphate, FRET, fluorescence resonance energy transfer, FP, fluorescent protein

PY - 2006

Y1 - 2006

N2 - Genetically-encoded fluorescence resonance energy transfer (FRET) sensors for phosphate (Pi) (FLIPPi) were engineered by fusing a predicted Synechococcus phosphate-binding protein (PiBP) to eCFP and Venus. Purified fluorescent indicator protein for inorganic phosphate (FLIPPi), in which the fluorophores are attached to the same PiBP lobe, shows Pi-dependent increases in FRET efficiency. FLIPPi affinity mutants cover Pi changes over eight orders of magnitude. COS-7 cells co-expressing a low-affinity FLIPPi and a Na+/Pi co-transporter exhibited FRET changes when perfused with 100µM Pi, demonstrating concentrative Pi uptake by PiT2. FLIPPi sensors are suitable for real-time monitoring of Pi metabolism in living cells, providing a new tool for fluxomics, analysis of pathophysiology or changes of Pi during cell migration.

AB - Genetically-encoded fluorescence resonance energy transfer (FRET) sensors for phosphate (Pi) (FLIPPi) were engineered by fusing a predicted Synechococcus phosphate-binding protein (PiBP) to eCFP and Venus. Purified fluorescent indicator protein for inorganic phosphate (FLIPPi), in which the fluorophores are attached to the same PiBP lobe, shows Pi-dependent increases in FRET efficiency. FLIPPi affinity mutants cover Pi changes over eight orders of magnitude. COS-7 cells co-expressing a low-affinity FLIPPi and a Na+/Pi co-transporter exhibited FRET changes when perfused with 100µM Pi, demonstrating concentrative Pi uptake by PiT2. FLIPPi sensors are suitable for real-time monitoring of Pi metabolism in living cells, providing a new tool for fluxomics, analysis of pathophysiology or changes of Pi during cell migration.

KW - Former LIFE faculty

KW - Fluorescence energy transfer, Phosphate starvation, Biosensor, Synechococcus

U2 - 10.1016/j.febslet.2006.09.048

DO - 10.1016/j.febslet.2006.09.048

M3 - Journal article

C2 - 17034793

VL - 580

SP - 5885

EP - 5893

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 25

ER -

ID: 8042551