A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures

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A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures. / Christiansen, G B; Harbak, Barbara; Hede, S E; Gouliaev, A H; Olsen, Lars; Frydenvang, K; Egebjerg, J; Kastrup, J S; Holm, M M.

In: Neuroscience, Vol. 310, 03.12.2015, p. 709-22.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Christiansen, GB, Harbak, B, Hede, SE, Gouliaev, AH, Olsen, L, Frydenvang, K, Egebjerg, J, Kastrup, JS & Holm, MM 2015, 'A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures', Neuroscience, vol. 310, pp. 709-22. https://doi.org/10.1016/j.neuroscience.2015.09.073

APA

Christiansen, G. B., Harbak, B., Hede, S. E., Gouliaev, A. H., Olsen, L., Frydenvang, K., ... Holm, M. M. (2015). A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures. Neuroscience, 310, 709-22. https://doi.org/10.1016/j.neuroscience.2015.09.073

Vancouver

Christiansen GB, Harbak B, Hede SE, Gouliaev AH, Olsen L, Frydenvang K et al. A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures. Neuroscience. 2015 Dec 3;310:709-22. https://doi.org/10.1016/j.neuroscience.2015.09.073

Author

Christiansen, G B ; Harbak, Barbara ; Hede, S E ; Gouliaev, A H ; Olsen, Lars ; Frydenvang, K ; Egebjerg, J ; Kastrup, J S ; Holm, M M. / A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures. In: Neuroscience. 2015 ; Vol. 310. pp. 709-22.

Bibtex

@article{4e839b6dd1f54d138725d987631b7115,
title = "A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures",
abstract = "Positive allosteric modulators (PAMs) of 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA) receptors receive increasing interest as therapeutic drugs and have long served as important experimental tools in the study of the molecular mechanisms underlying glutamate-mediated neurotransmission. The aim of this study was to investigate functional and structural aspects of a novel analog of the AMPA receptor PAM cyclothiazide (CTZ) on recombinant and native glutamate receptors. We expressed rat GluA4flip and flop in Xenopus oocytes and characterized NS1376 and CTZ under two-electrode voltage-clamp. The dose-response analyses revealed dual effects of NS1376. The modulator induced 30-fold and 42-fold reductions in glutamate potency and increased the glutamate efficacy by 3.2-fold and 5.3-fold at GluA4flip and GluA4flop, respectively. Rapid application of glutamate to excised outside-out patches showed that NS1376 markedly attenuated desensitization, supporting the increased efficacy observed in the oocytes. Furthermore, when applied to acutely isolated mouse brain slices, NS1376 reduced the field excitatory postsynaptic potentials (fEPSPs) in the hippocampus to 51.6 ± 4.3{\%} of baseline, likely as a consequence of reduced glutamate potency. However, the modulator displayed no effects on a sub-maximal long-term potentiation (LTP) protocol. We confirmed that CTZ increases presynaptic transmitter release, a property which was not shared by NS1376. Finally, we obtained detailed molecular information through X-ray structures, docking and molecular dynamics, which revealed that NS1376 interacts at the dimer interface of the ligand-binding domain in a manner overall similar to CTZ. NS1376 reveals that minor structural changes in CTZ can result in an altered modulatory profile, both enhancing agonist efficacy while markedly reducing agonist potency. These unique properties add new aspects to the complexity of allosteric modulations in neuronal systems.",
author = "Christiansen, {G B} and Barbara Harbak and Hede, {S E} and Gouliaev, {A H} and Lars Olsen and K Frydenvang and J Egebjerg and Kastrup, {J S} and Holm, {M M}",
note = "Copyright {\circledC} 2015 IBRO. Published by Elsevier Ltd. All rights reserved.",
year = "2015",
month = "12",
day = "3",
doi = "10.1016/j.neuroscience.2015.09.073",
language = "English",
volume = "310",
pages = "709--22",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - A novel dualistic profile of an allosteric AMPA receptor modulator identified through studies on recombinant receptors, mouse hippocampal synapses and crystal structures

AU - Christiansen, G B

AU - Harbak, Barbara

AU - Hede, S E

AU - Gouliaev, A H

AU - Olsen, Lars

AU - Frydenvang, K

AU - Egebjerg, J

AU - Kastrup, J S

AU - Holm, M M

N1 - Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

PY - 2015/12/3

Y1 - 2015/12/3

N2 - Positive allosteric modulators (PAMs) of 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA) receptors receive increasing interest as therapeutic drugs and have long served as important experimental tools in the study of the molecular mechanisms underlying glutamate-mediated neurotransmission. The aim of this study was to investigate functional and structural aspects of a novel analog of the AMPA receptor PAM cyclothiazide (CTZ) on recombinant and native glutamate receptors. We expressed rat GluA4flip and flop in Xenopus oocytes and characterized NS1376 and CTZ under two-electrode voltage-clamp. The dose-response analyses revealed dual effects of NS1376. The modulator induced 30-fold and 42-fold reductions in glutamate potency and increased the glutamate efficacy by 3.2-fold and 5.3-fold at GluA4flip and GluA4flop, respectively. Rapid application of glutamate to excised outside-out patches showed that NS1376 markedly attenuated desensitization, supporting the increased efficacy observed in the oocytes. Furthermore, when applied to acutely isolated mouse brain slices, NS1376 reduced the field excitatory postsynaptic potentials (fEPSPs) in the hippocampus to 51.6 ± 4.3% of baseline, likely as a consequence of reduced glutamate potency. However, the modulator displayed no effects on a sub-maximal long-term potentiation (LTP) protocol. We confirmed that CTZ increases presynaptic transmitter release, a property which was not shared by NS1376. Finally, we obtained detailed molecular information through X-ray structures, docking and molecular dynamics, which revealed that NS1376 interacts at the dimer interface of the ligand-binding domain in a manner overall similar to CTZ. NS1376 reveals that minor structural changes in CTZ can result in an altered modulatory profile, both enhancing agonist efficacy while markedly reducing agonist potency. These unique properties add new aspects to the complexity of allosteric modulations in neuronal systems.

AB - Positive allosteric modulators (PAMs) of 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA) receptors receive increasing interest as therapeutic drugs and have long served as important experimental tools in the study of the molecular mechanisms underlying glutamate-mediated neurotransmission. The aim of this study was to investigate functional and structural aspects of a novel analog of the AMPA receptor PAM cyclothiazide (CTZ) on recombinant and native glutamate receptors. We expressed rat GluA4flip and flop in Xenopus oocytes and characterized NS1376 and CTZ under two-electrode voltage-clamp. The dose-response analyses revealed dual effects of NS1376. The modulator induced 30-fold and 42-fold reductions in glutamate potency and increased the glutamate efficacy by 3.2-fold and 5.3-fold at GluA4flip and GluA4flop, respectively. Rapid application of glutamate to excised outside-out patches showed that NS1376 markedly attenuated desensitization, supporting the increased efficacy observed in the oocytes. Furthermore, when applied to acutely isolated mouse brain slices, NS1376 reduced the field excitatory postsynaptic potentials (fEPSPs) in the hippocampus to 51.6 ± 4.3% of baseline, likely as a consequence of reduced glutamate potency. However, the modulator displayed no effects on a sub-maximal long-term potentiation (LTP) protocol. We confirmed that CTZ increases presynaptic transmitter release, a property which was not shared by NS1376. Finally, we obtained detailed molecular information through X-ray structures, docking and molecular dynamics, which revealed that NS1376 interacts at the dimer interface of the ligand-binding domain in a manner overall similar to CTZ. NS1376 reveals that minor structural changes in CTZ can result in an altered modulatory profile, both enhancing agonist efficacy while markedly reducing agonist potency. These unique properties add new aspects to the complexity of allosteric modulations in neuronal systems.

U2 - 10.1016/j.neuroscience.2015.09.073

DO - 10.1016/j.neuroscience.2015.09.073

M3 - Journal article

VL - 310

SP - 709

EP - 722

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

ER -

ID: 161555498