Antifungal metabolites from fungal endophytes of Pinus strobus

Research output: Contribution to journalJournal articleResearchpeer-review

Mark W Sumarah, Julie R Kesting, Dan Sørensen, J David Miller

The extracts of five foliar fungal endophytes isolated from Pinus strobus (eastern white pine) that showed antifungal activity in disc diffusion assays were selected for further study. From these strains, the aliphatic polyketide compound 1 and three related sesquiterpenes 2-4 were isolated and characterized. Compound 2 is reported for the first time as a natural product and the E/Z conformational isomers 3 and 4 were hitherto unknown. Additionally, the three known macrolides; pyrenophorol (5), dihydropyrenophorin (6), and pyrenophorin (7) were isolated and identified. Their structures were elucidated by spectroscopic analyses including 2D NMR, HRMS and by comparison to literature data where available. The isolated compounds 1, 2, and 5 were antifungal against both the rust Microbotryum violaceum and Saccharomyces cerevisae.
Original languageEnglish
JournalPhytochemistry
Volume72
Issue number14-15
Pages (from-to)1833-1837
ISSN0031-9422
DOIs
Publication statusPublished - 1 Oct 2011

Bibliographical note

Keywords: Eastern white pine; Pinus strobus; Pinaceae; Cronartium ribicola; Microbotryum violaceum; Endophyte; LC-MS-SPE/NMR

ID: 35410668