Bovine cumulus-oocyte disconnection in vitro

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Bovine cumulus-oocyte disconnection in vitro. / Maddox-Hyttel, Poul.

In: Brain Structure and Function (Print Edition), Vol. 176, 1987, p. 41-44.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Maddox-Hyttel, P 1987, 'Bovine cumulus-oocyte disconnection in vitro', Brain Structure and Function (Print Edition), vol. 176, pp. 41-44.

APA

Maddox-Hyttel, P. (1987). Bovine cumulus-oocyte disconnection in vitro. Brain Structure and Function (Print Edition), 176, 41-44.

Vancouver

Maddox-Hyttel P. Bovine cumulus-oocyte disconnection in vitro. Brain Structure and Function (Print Edition). 1987;176:41-44.

Author

Maddox-Hyttel, Poul. / Bovine cumulus-oocyte disconnection in vitro. In: Brain Structure and Function (Print Edition). 1987 ; Vol. 176. pp. 41-44.

Bibtex

@article{4b47b1a0a1c211ddb6ae000ea68e967b,
title = "Bovine cumulus-oocyte disconnection in vitro",
abstract = "Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.",
keywords = "Former LIFE faculty, Bovine, Oocyte maturation, Meiosis, Gap junction, Ultrastructure",
author = "Poul Maddox-Hyttel",
year = "1987",
language = "English",
volume = "176",
pages = "41--44",
journal = "Brain Structure and Function (Print Edition)",
issn = "1863-2653",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Bovine cumulus-oocyte disconnection in vitro

AU - Maddox-Hyttel, Poul

PY - 1987

Y1 - 1987

N2 - Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.

AB - Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.

KW - Former LIFE faculty

KW - Bovine

KW - Oocyte maturation

KW - Meiosis

KW - Gap junction

KW - Ultrastructure

M3 - Journal article

VL - 176

SP - 41

EP - 44

JO - Brain Structure and Function (Print Edition)

JF - Brain Structure and Function (Print Edition)

SN - 1863-2653

ER -

ID: 8067538