Bovine cumulus-oocyte disconnection in vitro

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Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.
Original languageEnglish
JournalBrain Structure and Function (Print Edition)
Volume176
Pages (from-to)41-44
Number of pages4
ISSN1863-2653
Publication statusPublished - 1987

    Research areas

  • Former LIFE faculty - Bovine, Oocyte maturation, Meiosis, Gap junction, Ultrastructure

ID: 8067538