Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats

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Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats. / Ghorbani, Marie Louise; Nyborg, Niels C B; Fjalland, Bjarne; Sheykhzade, Majid.

In: International Journal of Endocrinology, Vol. 2013, 2013, p. Article ID 532850.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ghorbani, ML, Nyborg, NCB, Fjalland, B & Sheykhzade, M 2013, 'Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats', International Journal of Endocrinology, vol. 2013, pp. Article ID 532850. https://doi.org/10.1155/2013/532850

APA

Ghorbani, M. L., Nyborg, N. C. B., Fjalland, B., & Sheykhzade, M. (2013). Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats. International Journal of Endocrinology, 2013, Article ID 532850. https://doi.org/10.1155/2013/532850

Vancouver

Ghorbani ML, Nyborg NCB, Fjalland B, Sheykhzade M. Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats. International Journal of Endocrinology. 2013;2013:Article ID 532850. https://doi.org/10.1155/2013/532850

Author

Ghorbani, Marie Louise ; Nyborg, Niels C B ; Fjalland, Bjarne ; Sheykhzade, Majid. / Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats. In: International Journal of Endocrinology. 2013 ; Vol. 2013. pp. Article ID 532850.

Bibtex

@article{f1083d1aeb654e8eba922cd9795e8709,
title = "Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats",
abstract = "The aim of the present study was to examine the calcium activity of C8-T5 dorsal root ganglion (DRG) neurons from Zucker diabetic fatty rats. In total, 8 diabetic ZDF fatty animals and 8 age-matched control ZDF lean rats were employed in the study. C8-T5 dorsal root ganglia were isolated bilaterally from 14 to 18 weeks old rats, and a primary culture was prepared. Calcium activity was measured ratiometrically using the fluorescent Ca(2+)-indicator Fura-2 acetoxymethyl ester. All neurons were stimulated twice with 20¿mM¿K(+), followed by stimulation with either 0.3 or 0.5¿ µ M Capsaicin, alone or in combination with algogenic chemicals (bradykinin, serotonin, prostaglandin E2 (all 10(-5)¿M), and adenosine (10(-3)¿M)) at pH 7.4 and 6.0. Neurons from diabetic animals exhibited an overall increased response to stimulation with 20¿mM¿K(+) compared to neurons from control. Stimulation with Capsaicin alone caused an augmented response in neurons from diabetic animals compared to control animals. When stimulated with a combination of Capsaicin and algogenic chemicals, no differences between the two groups of neurons were measured, neither at pH 7.4 nor 6.0. In conclusion, diabetes-induced alterations in calcium activity of the DRG neurons were found, potentially indicating altered neuronal responses during myocardial ischemia.",
author = "Ghorbani, {Marie Louise} and Nyborg, {Niels C B} and Bjarne Fjalland and Majid Sheykhzade",
year = "2013",
doi = "10.1155/2013/532850",
language = "English",
volume = "2013",
pages = "Article ID 532850",
journal = "International Journal of Endocrinology",
issn = "1687-8337",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats

AU - Ghorbani, Marie Louise

AU - Nyborg, Niels C B

AU - Fjalland, Bjarne

AU - Sheykhzade, Majid

PY - 2013

Y1 - 2013

N2 - The aim of the present study was to examine the calcium activity of C8-T5 dorsal root ganglion (DRG) neurons from Zucker diabetic fatty rats. In total, 8 diabetic ZDF fatty animals and 8 age-matched control ZDF lean rats were employed in the study. C8-T5 dorsal root ganglia were isolated bilaterally from 14 to 18 weeks old rats, and a primary culture was prepared. Calcium activity was measured ratiometrically using the fluorescent Ca(2+)-indicator Fura-2 acetoxymethyl ester. All neurons were stimulated twice with 20¿mM¿K(+), followed by stimulation with either 0.3 or 0.5¿ µ M Capsaicin, alone or in combination with algogenic chemicals (bradykinin, serotonin, prostaglandin E2 (all 10(-5)¿M), and adenosine (10(-3)¿M)) at pH 7.4 and 6.0. Neurons from diabetic animals exhibited an overall increased response to stimulation with 20¿mM¿K(+) compared to neurons from control. Stimulation with Capsaicin alone caused an augmented response in neurons from diabetic animals compared to control animals. When stimulated with a combination of Capsaicin and algogenic chemicals, no differences between the two groups of neurons were measured, neither at pH 7.4 nor 6.0. In conclusion, diabetes-induced alterations in calcium activity of the DRG neurons were found, potentially indicating altered neuronal responses during myocardial ischemia.

AB - The aim of the present study was to examine the calcium activity of C8-T5 dorsal root ganglion (DRG) neurons from Zucker diabetic fatty rats. In total, 8 diabetic ZDF fatty animals and 8 age-matched control ZDF lean rats were employed in the study. C8-T5 dorsal root ganglia were isolated bilaterally from 14 to 18 weeks old rats, and a primary culture was prepared. Calcium activity was measured ratiometrically using the fluorescent Ca(2+)-indicator Fura-2 acetoxymethyl ester. All neurons were stimulated twice with 20¿mM¿K(+), followed by stimulation with either 0.3 or 0.5¿ µ M Capsaicin, alone or in combination with algogenic chemicals (bradykinin, serotonin, prostaglandin E2 (all 10(-5)¿M), and adenosine (10(-3)¿M)) at pH 7.4 and 6.0. Neurons from diabetic animals exhibited an overall increased response to stimulation with 20¿mM¿K(+) compared to neurons from control. Stimulation with Capsaicin alone caused an augmented response in neurons from diabetic animals compared to control animals. When stimulated with a combination of Capsaicin and algogenic chemicals, no differences between the two groups of neurons were measured, neither at pH 7.4 nor 6.0. In conclusion, diabetes-induced alterations in calcium activity of the DRG neurons were found, potentially indicating altered neuronal responses during myocardial ischemia.

U2 - 10.1155/2013/532850

DO - 10.1155/2013/532850

M3 - Journal article

VL - 2013

SP - Article ID 532850

JO - International Journal of Endocrinology

JF - International Journal of Endocrinology

SN - 1687-8337

ER -

ID: 45665493