Canine specific ELISA for coagulation factor VII

Research output: Contribution to journalJournal articleResearchpeer-review

Tom Knudsen, Mads Kjelgaard-Hansen, Mikael Tranholm, Bo Wiinberg, Jes T. Clausen, Jens Jacob Hansen, Timothy C. Nichols, Marianne Kjalke, Asger Lundorff Jensen, Annemarie Thuri Kristensen

Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.
Original languageEnglish
JournalThe Veterinary Journal
Volume190
Issue number3
Pages (from-to)352-358
Number of pages7
ISSN1090-0233
DOIs
Publication statusPublished - 2011

ID: 35352398