Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome

Research output: Contribution to journalJournal articleResearch

Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)8, (AAGG)8, (AGGG)8, (ATAG)8 and (AG)15 oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.
Original languageEnglish
Issue number2
Pages (from-to)55-58
Number of pages4
Publication statusPublished - 2007

ID: 8078799