Direct microcomputer controlled determination of zinc in human serum by flow injection atomic absorption spectrometry
Research output: Contribution to journal › Journal article › Research › peer-review
Kirsten Wiese Simonsen, Bent Nielsen, Arne Jensen, Jan Rud Andersen
A procedure is described for the direct determination of zinc in human serum by fully automated, microcomputer controlled flow injection atomic absorption spectrometry (Fl-AAS). The Fl system is pumpless, using the negative pressure created by the nebuliser. It only consists of a three-way valve, programmable from the microcomputer, to control the sample volume. No pre-treatment of the samples is necessary. The limit of detection is 0.14 mg l–1, and only small amounts of serum (<150 µl) are needed for the analysis. The accuracy, as estimated by analysing sera with known amounts of zinc, is excellent, and the between batch precision is ca. 5% RSD. Peak-area measurements are used in the procedure; as a consequence the quantitation can be carried out by use of an aqueous calibration graph. The sample throughput is 40 h–1, making the procedure well suited for routine clinical analysis.
|Journal||Journal of Analytical Atomic Spectrometry|
|Number of pages||4|
|Publication status||Published - 1 Jan 1986|
- Former Faculty of Pharmaceutical Sciences - Zinc determination, Flow Injection , flame atomic absorption spectrometry, human serum, microcomputer control