Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1

Research output: Contribution to journalJournal articleResearchpeer-review

Rupali Vohra, Iswariyaraja Sridevi Gurubaran, Ulrik Henriksen, Linda Hildegaard Bergersen, Lene Juel Rasmussen, Claus Desler, Dorte Marie Skytt, Miriam Kolko

Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.

Original languageEnglish
JournalMitochondrion
Volume36
Pages (from-to)52-59
ISSN1567-7249
DOIs
Publication statusPublished - 2017

    Research areas

  • Antimycin A, Glutamate, Lactate, MIO-M1 cell line, Mitochondria, Müller cells

ID: 179048691