Effect of Ageing on the Passive and Active Tension and Pharmacodynamic Characteristics of Rat Coronary Arteries: Age-Dependent Increase in Sensitivity to 5-HT and K+

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Majid Sheykhzade, Anja Hviid Simonsen, Harrie C.M. Boonen, Emilie Middelbo Outzen, Niels Chresten Berg Nyborg

The influence of ageing on the passive and active tension and pharmacodynamic characteristics of intramural coronary arteries from 3-month-old and 2-year-old male Wistar rats was investigated using an isometric myograph. The passive vessel wall tension measured in Ca2+-free physiological salt solution at L0 was significantly greater in arteries from old rats (1.46 ± 0.10 Nm–1, n = 7) than in young rats (1.13 ± 0.13 Nm–1, n = 6). However, the maximal active tension at L0 was similar. The spontaneous myogenic tone was increased by age and the vasorelaxation induced by extracellular K+ was significantly higher in coronary arteries of old rats. The sensitivity (pD2) to 5-HT was significantly higher in arteries from old (6.43 ± 0.11, n = 22) than from young rats (6.16 ± 0.08, n = 29). Ketanserin induced a concentration-dependent rightward shift of the 5-HT concentration-response curve in arteries from both young and old rats. The slopes of the regression lines of the Schild plots were not significantly different from unity and the estimated pKB values for ketanserin were similar. In conclusion, ageing is associated with changes in passive mechanical characteristics as well as changes in pharmacological properties in rat coronary small arteries.
Original languageEnglish
JournalPharmacology
Volume90
Issue number3-4
Pages (from-to)160-168
Number of pages9
ISSN0031-7012
DOIs
Publication statusPublished - 22 Aug 2012

ID: 40349736