Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats

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Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats. / Stefkov, Gjoshe; Kulevanova, Svetlana; Miova, Biljana; Dinevska-Kjovkarovska, Suzana; Mølgaard, Per; Jäger, Anna K; Josefsen, Knud.

In: Pharmaceutical Biology, Vol. 49, No. 9, 01.09.2011, p. 885-892.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Stefkov, G, Kulevanova, S, Miova, B, Dinevska-Kjovkarovska, S, Mølgaard, P, Jäger, AK & Josefsen, K 2011, 'Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats', Pharmaceutical Biology, vol. 49, no. 9, pp. 885-892. https://doi.org/10.3109/13880209.2011.552187

APA

Stefkov, G., Kulevanova, S., Miova, B., Dinevska-Kjovkarovska, S., Mølgaard, P., Jäger, A. K., & Josefsen, K. (2011). Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats. Pharmaceutical Biology, 49(9), 885-892. https://doi.org/10.3109/13880209.2011.552187

Vancouver

Stefkov G, Kulevanova S, Miova B, Dinevska-Kjovkarovska S, Mølgaard P, Jäger AK et al. Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats. Pharmaceutical Biology. 2011 Sep 1;49(9):885-892. https://doi.org/10.3109/13880209.2011.552187

Author

Stefkov, Gjoshe ; Kulevanova, Svetlana ; Miova, Biljana ; Dinevska-Kjovkarovska, Suzana ; Mølgaard, Per ; Jäger, Anna K ; Josefsen, Knud. / Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats. In: Pharmaceutical Biology. 2011 ; Vol. 49, No. 9. pp. 885-892.

Bibtex

@article{bad0d87bdc81432d85e35d4795e8ad51,
title = "Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats",
abstract = "Context: The main objective of the study was to investigate the biochemical mechanism of the antidiabetic activities of the dry extracts of Teucrium polium L. ssp. capitatum (L.) Arcangeli (Lamiaceae), from Republic of Macedonia, traditionally used to treat diabetes. Materials and methods: Aerial parts of the plant were extracted in alcohol and freeze- or spray-dried, analyzed by high performance liquid chromatography (HPLC) and examined for insulinotropic effect in INS-1E cells in vitro. Their effect on blood glucose, lipids and carbohydrate-related enzymes was tested in normo- and streptozotocin hyperglycemic rats. Results and discussion: HPLC analyses revealed several flavonoids: luteolin, apigenin, cirsiliol, diosmetin, cirsimaritin and cirsilineol as both free aglycons and glycosides. The extract and mixture of commercial flavonoids showed a distinct insulinotropic effect on INS-1E cells at 500 µg/ml. Intragastric (i.g.) administration of identical doses of the extract (125¿mg/kg) in both normo- and hyperglycemic rats was more efficient in lowering the blood glucose than intraperitoneal injection (35{\%} vs. 24{\%} reduction) with highest effect (50{\%} reduction) 8¿h after administration. After 10 days of treatment, the magnitude of the effect was comparable to i.g. administration of 2.5¿mg/kg of glibenclamide (38{\%} reduction). No effect was seen on blood lipid profiles. In OGTT (oral glucose tolerance test), the extract lowered blood glucose levels by ~35{\%}. The treatment reduced hepatic glycogen and tended to normalize the activity of gluconeogenic enzymes. Conclusion: The results demonstrate that examined plant extracts contain flavonoids with insulinotropic and antihyperglycemic effects.",
keywords = "The Faculty of Pharmaceutical Sciences, The Faculty of Health and Medical Sciences",
author = "Gjoshe Stefkov and Svetlana Kulevanova and Biljana Miova and Suzana Dinevska-Kjovkarovska and Per M{\o}lgaard and J{\"a}ger, {Anna K} and Knud Josefsen",
note = "Keywords: golden germander, plant extract, INS-1E cells, STZ-rats, diabetes, insulinotropic, hypoglycemic, R. Macedonia",
year = "2011",
month = "9",
day = "1",
doi = "10.3109/13880209.2011.552187",
language = "English",
volume = "49",
pages = "885--892",
journal = "Pharmaceutical Biology",
issn = "1388-0209",
publisher = "Taylor & Francis",
number = "9",

}

RIS

TY - JOUR

T1 - Effects of Teucrium polium spp. capitatum flavonoids on the lipid and carbohydrate metabolism in rats

AU - Stefkov, Gjoshe

AU - Kulevanova, Svetlana

AU - Miova, Biljana

AU - Dinevska-Kjovkarovska, Suzana

AU - Mølgaard, Per

AU - Jäger, Anna K

AU - Josefsen, Knud

N1 - Keywords: golden germander, plant extract, INS-1E cells, STZ-rats, diabetes, insulinotropic, hypoglycemic, R. Macedonia

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Context: The main objective of the study was to investigate the biochemical mechanism of the antidiabetic activities of the dry extracts of Teucrium polium L. ssp. capitatum (L.) Arcangeli (Lamiaceae), from Republic of Macedonia, traditionally used to treat diabetes. Materials and methods: Aerial parts of the plant were extracted in alcohol and freeze- or spray-dried, analyzed by high performance liquid chromatography (HPLC) and examined for insulinotropic effect in INS-1E cells in vitro. Their effect on blood glucose, lipids and carbohydrate-related enzymes was tested in normo- and streptozotocin hyperglycemic rats. Results and discussion: HPLC analyses revealed several flavonoids: luteolin, apigenin, cirsiliol, diosmetin, cirsimaritin and cirsilineol as both free aglycons and glycosides. The extract and mixture of commercial flavonoids showed a distinct insulinotropic effect on INS-1E cells at 500 µg/ml. Intragastric (i.g.) administration of identical doses of the extract (125¿mg/kg) in both normo- and hyperglycemic rats was more efficient in lowering the blood glucose than intraperitoneal injection (35% vs. 24% reduction) with highest effect (50% reduction) 8¿h after administration. After 10 days of treatment, the magnitude of the effect was comparable to i.g. administration of 2.5¿mg/kg of glibenclamide (38% reduction). No effect was seen on blood lipid profiles. In OGTT (oral glucose tolerance test), the extract lowered blood glucose levels by ~35%. The treatment reduced hepatic glycogen and tended to normalize the activity of gluconeogenic enzymes. Conclusion: The results demonstrate that examined plant extracts contain flavonoids with insulinotropic and antihyperglycemic effects.

AB - Context: The main objective of the study was to investigate the biochemical mechanism of the antidiabetic activities of the dry extracts of Teucrium polium L. ssp. capitatum (L.) Arcangeli (Lamiaceae), from Republic of Macedonia, traditionally used to treat diabetes. Materials and methods: Aerial parts of the plant were extracted in alcohol and freeze- or spray-dried, analyzed by high performance liquid chromatography (HPLC) and examined for insulinotropic effect in INS-1E cells in vitro. Their effect on blood glucose, lipids and carbohydrate-related enzymes was tested in normo- and streptozotocin hyperglycemic rats. Results and discussion: HPLC analyses revealed several flavonoids: luteolin, apigenin, cirsiliol, diosmetin, cirsimaritin and cirsilineol as both free aglycons and glycosides. The extract and mixture of commercial flavonoids showed a distinct insulinotropic effect on INS-1E cells at 500 µg/ml. Intragastric (i.g.) administration of identical doses of the extract (125¿mg/kg) in both normo- and hyperglycemic rats was more efficient in lowering the blood glucose than intraperitoneal injection (35% vs. 24% reduction) with highest effect (50% reduction) 8¿h after administration. After 10 days of treatment, the magnitude of the effect was comparable to i.g. administration of 2.5¿mg/kg of glibenclamide (38% reduction). No effect was seen on blood lipid profiles. In OGTT (oral glucose tolerance test), the extract lowered blood glucose levels by ~35%. The treatment reduced hepatic glycogen and tended to normalize the activity of gluconeogenic enzymes. Conclusion: The results demonstrate that examined plant extracts contain flavonoids with insulinotropic and antihyperglycemic effects.

KW - The Faculty of Pharmaceutical Sciences

KW - The Faculty of Health and Medical Sciences

U2 - 10.3109/13880209.2011.552187

DO - 10.3109/13880209.2011.552187

M3 - Journal article

VL - 49

SP - 885

EP - 892

JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

SN - 1388-0209

IS - 9

ER -

ID: 34338487