Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling

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Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling. / Sørensen, Amalie Møller; Hansen, Cecilie Hurup; Bonomo, Silvia; Olsen, Lars; Jørgensen, Flemming Steen; Weisser, Johan Juhl; Kretschmann, Andreas Christopher; Styrishave, Bjarne.

In: Toxicology in Vitro, Vol. 34, 19.03.2016, p. 71-80.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Sørensen, AM, Hansen, CH, Bonomo, S, Olsen, L, Jørgensen, FS, Weisser, JJ, Kretschmann, AC & Styrishave, B 2016, 'Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling', Toxicology in Vitro, vol. 34, pp. 71-80. https://doi.org/10.1016/j.tiv.2016.03.007

APA

Sørensen, A. M., Hansen, C. H., Bonomo, S., Olsen, L., Jørgensen, F. S., Weisser, J. J., ... Styrishave, B. (2016). Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling. Toxicology in Vitro, 34, 71-80. https://doi.org/10.1016/j.tiv.2016.03.007

Vancouver

Sørensen AM, Hansen CH, Bonomo S, Olsen L, Jørgensen FS, Weisser JJ et al. Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling. Toxicology in Vitro. 2016 Mar 19;34:71-80. https://doi.org/10.1016/j.tiv.2016.03.007

Author

Sørensen, Amalie Møller ; Hansen, Cecilie Hurup ; Bonomo, Silvia ; Olsen, Lars ; Jørgensen, Flemming Steen ; Weisser, Johan Juhl ; Kretschmann, Andreas Christopher ; Styrishave, Bjarne. / Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling. In: Toxicology in Vitro. 2016 ; Vol. 34. pp. 71-80.

Bibtex

@article{e2b927656c9049ccb3c49677c9ace5b0,
title = "Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling",
abstract = "Enantiomers possess different pharmacokinetic and pharmacodynamic properties and this may not only influence the therapeutic effect of a drug but also its toxicological effects. In the present work we investigated the potential enantioselective endocrine disrupting effects of omeprazole (OME) and its two enantiomers on the human steroidogenesis using the H295R cell line. Differences in production of 16 steroid hormones were analyzed using LC-MS/MS. Additionally, to evaluate the differences in binding modes of these enantiomers, docking and molecular dynamics (MD) simulations of S-omeprazole (S-OME) and R-omeprazole (R-OME) in CYP17A1, CYP19A1 and CYP21A2 were carried out. Exposing H295R cells to OME and its enantiomers resulted in an increase of progesterone (PRO) and 17α-hydroxy-progesterone (OH-PRO) levels. At the same time, a decrease in the corticosteroid and androgen synthesis was observed, indicating inhibition of CYP21A2 and CYP17A1. In both cases, the effect of R-OME was smaller compared to that of the S-OME and a certain degree of enantioselectivity of CYP17A1 and CYP21A2 was suggested. Docking indicated that the N-containing rings of OME possibly could interact with the iron atom of the heme for S-OME in CYP17A1 and S- and R-OME in CYP21A2. However, density functional theory calculations suggest that the direct N-Fe interaction is weak. The study demonstrates enantioselective differences in the endocrine disrupting potential of chiral drugs such as omeprazole. These findings may have potential implications for drug safety and drug design.",
author = "S{\o}rensen, {Amalie M{\o}ller} and Hansen, {Cecilie Hurup} and Silvia Bonomo and Lars Olsen and J{\o}rgensen, {Flemming Steen} and Weisser, {Johan Juhl} and Kretschmann, {Andreas Christopher} and Bjarne Styrishave",
note = "Copyright {\circledC} 2016 Elsevier B.V. All rights reserved.",
year = "2016",
month = "3",
day = "19",
doi = "10.1016/j.tiv.2016.03.007",
language = "English",
volume = "34",
pages = "71--80",
journal = "Toxicology in Vitro",
issn = "0887-2333",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Enantioselective endocrine disrupting effects of omeprazole studied in the H295R cell assay and by molecular modeling

AU - Sørensen, Amalie Møller

AU - Hansen, Cecilie Hurup

AU - Bonomo, Silvia

AU - Olsen, Lars

AU - Jørgensen, Flemming Steen

AU - Weisser, Johan Juhl

AU - Kretschmann, Andreas Christopher

AU - Styrishave, Bjarne

N1 - Copyright © 2016 Elsevier B.V. All rights reserved.

PY - 2016/3/19

Y1 - 2016/3/19

N2 - Enantiomers possess different pharmacokinetic and pharmacodynamic properties and this may not only influence the therapeutic effect of a drug but also its toxicological effects. In the present work we investigated the potential enantioselective endocrine disrupting effects of omeprazole (OME) and its two enantiomers on the human steroidogenesis using the H295R cell line. Differences in production of 16 steroid hormones were analyzed using LC-MS/MS. Additionally, to evaluate the differences in binding modes of these enantiomers, docking and molecular dynamics (MD) simulations of S-omeprazole (S-OME) and R-omeprazole (R-OME) in CYP17A1, CYP19A1 and CYP21A2 were carried out. Exposing H295R cells to OME and its enantiomers resulted in an increase of progesterone (PRO) and 17α-hydroxy-progesterone (OH-PRO) levels. At the same time, a decrease in the corticosteroid and androgen synthesis was observed, indicating inhibition of CYP21A2 and CYP17A1. In both cases, the effect of R-OME was smaller compared to that of the S-OME and a certain degree of enantioselectivity of CYP17A1 and CYP21A2 was suggested. Docking indicated that the N-containing rings of OME possibly could interact with the iron atom of the heme for S-OME in CYP17A1 and S- and R-OME in CYP21A2. However, density functional theory calculations suggest that the direct N-Fe interaction is weak. The study demonstrates enantioselective differences in the endocrine disrupting potential of chiral drugs such as omeprazole. These findings may have potential implications for drug safety and drug design.

AB - Enantiomers possess different pharmacokinetic and pharmacodynamic properties and this may not only influence the therapeutic effect of a drug but also its toxicological effects. In the present work we investigated the potential enantioselective endocrine disrupting effects of omeprazole (OME) and its two enantiomers on the human steroidogenesis using the H295R cell line. Differences in production of 16 steroid hormones were analyzed using LC-MS/MS. Additionally, to evaluate the differences in binding modes of these enantiomers, docking and molecular dynamics (MD) simulations of S-omeprazole (S-OME) and R-omeprazole (R-OME) in CYP17A1, CYP19A1 and CYP21A2 were carried out. Exposing H295R cells to OME and its enantiomers resulted in an increase of progesterone (PRO) and 17α-hydroxy-progesterone (OH-PRO) levels. At the same time, a decrease in the corticosteroid and androgen synthesis was observed, indicating inhibition of CYP21A2 and CYP17A1. In both cases, the effect of R-OME was smaller compared to that of the S-OME and a certain degree of enantioselectivity of CYP17A1 and CYP21A2 was suggested. Docking indicated that the N-containing rings of OME possibly could interact with the iron atom of the heme for S-OME in CYP17A1 and S- and R-OME in CYP21A2. However, density functional theory calculations suggest that the direct N-Fe interaction is weak. The study demonstrates enantioselective differences in the endocrine disrupting potential of chiral drugs such as omeprazole. These findings may have potential implications for drug safety and drug design.

U2 - 10.1016/j.tiv.2016.03.007

DO - 10.1016/j.tiv.2016.03.007

M3 - Journal article

VL - 34

SP - 71

EP - 80

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

ER -

ID: 161633884