Essential Roles of Lactate in Müller Cell Survival and Function

Research output: Contribution to journalJournal articleResearchpeer-review

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Essential Roles of Lactate in Müller Cell Survival and Function. / Vohra, Rupali; Aldana Garcia, Blanca Irene; Skytt, Dorte M; Freude, Kristine; Waagepetersen, Helle S.; Bergersen, Linda Hildegard; Kolko, Miriam.

In: Molecular Neurobiology, Vol. 55, No. 12, 2018, p. 9108-9121.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Vohra, R, Aldana Garcia, BI, Skytt, DM, Freude, K, Waagepetersen, HS, Bergersen, LH & Kolko, M 2018, 'Essential Roles of Lactate in Müller Cell Survival and Function', Molecular Neurobiology, vol. 55, no. 12, pp. 9108-9121. https://doi.org/10.1007/s12035-018-1056-2

APA

Vohra, R., Aldana Garcia, B. I., Skytt, D. M., Freude, K., Waagepetersen, H. S., Bergersen, L. H., & Kolko, M. (2018). Essential Roles of Lactate in Müller Cell Survival and Function. Molecular Neurobiology, 55(12), 9108-9121. https://doi.org/10.1007/s12035-018-1056-2

Vancouver

Vohra R, Aldana Garcia BI, Skytt DM, Freude K, Waagepetersen HS, Bergersen LH et al. Essential Roles of Lactate in Müller Cell Survival and Function. Molecular Neurobiology. 2018;55(12):9108-9121. https://doi.org/10.1007/s12035-018-1056-2

Author

Vohra, Rupali ; Aldana Garcia, Blanca Irene ; Skytt, Dorte M ; Freude, Kristine ; Waagepetersen, Helle S. ; Bergersen, Linda Hildegard ; Kolko, Miriam. / Essential Roles of Lactate in Müller Cell Survival and Function. In: Molecular Neurobiology. 2018 ; Vol. 55, No. 12. pp. 9108-9121.

Bibtex

@article{618ba63236574a169b3d9b73edde9afd,
title = "Essential Roles of Lactate in M{\"u}ller Cell Survival and Function",
abstract = "M{\"u}ller cells are pivotal in sustaining retinal ganglion cells, and an intact energy metabolism is essential for upholding M{\"u}ller cell functions. The present study aimed to investigate the impact of lactate on M{\"u}ller cell survival and function. Primary mice M{\"u}ller cells and human M{\"u}ller cell lines (MIO-M1) were treated with or without lactate (10 or 20 mM) for 2 and 24 hours. Simultaneously, M{\"u}ller cells were incubated with or without 6 mM of glucose. L-lactate exposure increased M{\"u}ller cell survival independently of the presence of glucose. This effect was abolished by the addition of the monocarboxylate inhibitor 4-cinnamic acid to the treatment media, whereas survival continued to increase in response to addition of D-lactate during glucose restriction. ATP levels decreased over time in MIO-M1 cells and remained stable over time in primary M{\"u}ller cells. Lactate was preferably metabolized in MIO-M1 cells compared to glucose, and 10 mM of L-Lactate exposure prevented complete glycogen depletion in MIO-M1 cells. Glutamate uptake increased after 2 hours and decreased after 24 hours in glucose-restricted M{\"u}ller cells compared to cells with glucose supplement. The addition of 10 mM of lactate to the treatment media increased glutamate uptake in glucose supplemented and restricted cells. In conclusion, lactate is a key component in maintaining M{\"u}ller cell survival and function. Hence, lactate administration may be of great future interest, ultimately leading to novel therapies to rescue retinal ganglion cells.",
author = "Rupali Vohra and {Aldana Garcia}, {Blanca Irene} and Skytt, {Dorte M} and Kristine Freude and Waagepetersen, {Helle S.} and Bergersen, {Linda Hildegard} and Miriam Kolko",
year = "2018",
doi = "10.1007/s12035-018-1056-2",
language = "English",
volume = "55",
pages = "9108--9121",
journal = "Molecular Neurobiology",
issn = "0893-7648",
publisher = "Springer",
number = "12",

}

RIS

TY - JOUR

T1 - Essential Roles of Lactate in Müller Cell Survival and Function

AU - Vohra, Rupali

AU - Aldana Garcia, Blanca Irene

AU - Skytt, Dorte M

AU - Freude, Kristine

AU - Waagepetersen, Helle S.

AU - Bergersen, Linda Hildegard

AU - Kolko, Miriam

PY - 2018

Y1 - 2018

N2 - Müller cells are pivotal in sustaining retinal ganglion cells, and an intact energy metabolism is essential for upholding Müller cell functions. The present study aimed to investigate the impact of lactate on Müller cell survival and function. Primary mice Müller cells and human Müller cell lines (MIO-M1) were treated with or without lactate (10 or 20 mM) for 2 and 24 hours. Simultaneously, Müller cells were incubated with or without 6 mM of glucose. L-lactate exposure increased Müller cell survival independently of the presence of glucose. This effect was abolished by the addition of the monocarboxylate inhibitor 4-cinnamic acid to the treatment media, whereas survival continued to increase in response to addition of D-lactate during glucose restriction. ATP levels decreased over time in MIO-M1 cells and remained stable over time in primary Müller cells. Lactate was preferably metabolized in MIO-M1 cells compared to glucose, and 10 mM of L-Lactate exposure prevented complete glycogen depletion in MIO-M1 cells. Glutamate uptake increased after 2 hours and decreased after 24 hours in glucose-restricted Müller cells compared to cells with glucose supplement. The addition of 10 mM of lactate to the treatment media increased glutamate uptake in glucose supplemented and restricted cells. In conclusion, lactate is a key component in maintaining Müller cell survival and function. Hence, lactate administration may be of great future interest, ultimately leading to novel therapies to rescue retinal ganglion cells.

AB - Müller cells are pivotal in sustaining retinal ganglion cells, and an intact energy metabolism is essential for upholding Müller cell functions. The present study aimed to investigate the impact of lactate on Müller cell survival and function. Primary mice Müller cells and human Müller cell lines (MIO-M1) were treated with or without lactate (10 or 20 mM) for 2 and 24 hours. Simultaneously, Müller cells were incubated with or without 6 mM of glucose. L-lactate exposure increased Müller cell survival independently of the presence of glucose. This effect was abolished by the addition of the monocarboxylate inhibitor 4-cinnamic acid to the treatment media, whereas survival continued to increase in response to addition of D-lactate during glucose restriction. ATP levels decreased over time in MIO-M1 cells and remained stable over time in primary Müller cells. Lactate was preferably metabolized in MIO-M1 cells compared to glucose, and 10 mM of L-Lactate exposure prevented complete glycogen depletion in MIO-M1 cells. Glutamate uptake increased after 2 hours and decreased after 24 hours in glucose-restricted Müller cells compared to cells with glucose supplement. The addition of 10 mM of lactate to the treatment media increased glutamate uptake in glucose supplemented and restricted cells. In conclusion, lactate is a key component in maintaining Müller cell survival and function. Hence, lactate administration may be of great future interest, ultimately leading to novel therapies to rescue retinal ganglion cells.

U2 - 10.1007/s12035-018-1056-2

DO - 10.1007/s12035-018-1056-2

M3 - Journal article

C2 - 29644598

VL - 55

SP - 9108

EP - 9121

JO - Molecular Neurobiology

JF - Molecular Neurobiology

SN - 0893-7648

IS - 12

ER -

ID: 195472790