Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs

Research output: Contribution to journalJournal article

  • R. Rimhanen-Finne
  • Heidi Larsen Enemark
  • J. Kolehmainen
  • P. Toropainen
  • M.L. Hänninen
The performance of immunoflourscence microscopy (IF) and enzyme-linked immunosorbent assay (ELISA) in canine feces was evaluated. IF and Cryptosporidium ELISA detected 105 oocysts/g, while the detection limit for Guardia ELISA was 104 cysts/g. The Cryptosporidium ELISA showed 94% specificity but only 71% sensitivity. The Guardia ELISA correlated well with IF (sensitivity 100%, specificity 96%) and was capable of detecting animal specific Guardia duodenalis genotypes.
Original languageEnglish
JournalVeterinary Parasitology
Issue number3-4
Pages (from-to)345-348
Number of pages4
Publication statusPublished - 2007

ID: 8073058