Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs

Research output: Contribution to journalJournal articleResearchpeer-review

R. Rimhanen-Finne, Heidi Larsen Enemark, J. Kolehmainen, P. Toropainen, M.L. Hänninen

The performance of immunoflourscence microscopy (IF) and enzyme-linked immunosorbent assay (ELISA) in canine feces was evaluated. IF and Cryptosporidium ELISA detected 105 oocysts/g, while the detection limit for Guardia ELISA was 104 cysts/g. The Cryptosporidium ELISA showed 94% specificity but only 71% sensitivity. The Guardia ELISA correlated well with IF (sensitivity 100%, specificity 96%) and was capable of detecting animal specific Guardia duodenalis genotypes.
Original languageEnglish
JournalVeterinary Parasitology
Volume145
Issue number3-4
Pages (from-to)345-348
Number of pages4
ISSN0304-4017
DOIs
Publication statusPublished - 2007

    Research areas

  • LIFE - Cryptosporidium, Guardia, Dog, IF, ELISA

ID: 8073058