Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1

Research output: Contribution to journalJournal articleResearchpeer-review

Nikolaj Kulahin, Christina Kasper, Ole Kristensen, Jette Sandholm Kastrup, Vladimir Berezin, Elisabeth Bock, Michael Gajhede

Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.
Original languageEnglish
JournalActa Crystallographica. Section F : Structural Biology and Crystallization Communications
Volume61
Issue numberPt 9
Pages (from-to)858-60
Number of pages2
ISSN1744-3091
DOIs
Publication statusPublished - 2005

Bibliographical note

Keywords: Animals; Crystallization; Crystallography, X-Ray; Fibronectins; Gene Expression; Immunoglobulins; Mice; Neural Cell Adhesion Molecule L1

ID: 21605053