Gene Expression Analysis Using Agilent DNA Microarrays

Research output: Chapter in Book/Report/Conference proceedingBook chapterEducation

Michael Stangegaard

Hybridization of labeled cDNA to microarrays is an intuitively simple and a vastly underestimated process. If it is not performed, optimized, and standardized with the same attention to detail as e.g., RNA amplification, information may be overlooked or even lost. Careful balancing of the amount of labeled cDNA added to each slide reduces dye-bias and slide to slide variation. Efficient mixing of the hybridization solution throughout the hybridization reaction increases signals several fold. The amount of near perfect target-probe hybrids may be reduced by efficient stringency washes of the hybridized microarray slides.
Original languageEnglish
Title of host publicationDNA Microarrays for Biomedical Research : Methods and Protocols
EditorsHans Martin Dufva
Number of pages13
Volume529
Place of PublicationSpring Street, New York, NY 10013, USA
PublisherSpringer Science+Business Media
Publication date2009
Edition1
Pages133-145
ISBN (Print)1064-3745, 978-1-934115-69-5
ISBN (Electronic)1940-6029, 978-1-59745-538-1
DOIs
Publication statusPublished - 2009
SeriesMethods in Molecular Biology

ID: 13974028