Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

Research output: Contribution to journalJournal articleResearchpeer-review

Lotte Gam Pedersen, J. Zhao, J. Yang, Preben Dybdahl Thomsen, H. Gregersen, J.M. Hasenkam, M. Smerup, H.D. Pedersen, Lisbeth Høier Olsen

The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.

Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.

The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.

Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

Original languageEnglish
JournalResearch in Veterinary Science
Issue number2
Pages (from-to)232-238
Number of pages7
Publication statusPublished - 2007

    Research areas

  • LIFE - Endothelin, Endothelin receptors, Mitral valve, Real-time PCR, Stress, Stretch

ID: 8045011