Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets
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The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.
Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.
The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.
Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.
|Journal||Research in Veterinary Science|
|Number of pages||7|
|Publication status||Published - 2007|
- LIFE - Endothelin, Endothelin receptors, Mitral valve, Real-time PCR, Stress, Stretch