LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries

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Bahareh Ghorbani, Palle Holmstrup, Lars Edvinsson, Kim A Kristiansen, Majid Sheykhzade, Bahareh Ghorbani, Palle Holmstrup, Lars Edvinsson, Kim A Kristiansen, Majid Sheykhzade

The purpose of our study was to examine if lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g.) modifies the vasomotor responses to Endothelin-1 (ET-1) and Sarafotoxin 6c (S6c) in rat coronary arteries. The arteries were studied directly or following organ culture for 24h in absence and presence of 2.5EU/ml LPS. The contractile responses of coronary arteries were investigated by using the selective ETB receptor agonist S6c (1 pM-0.3µM) and ET-1 (1 pM-0.3µM). The functional studies demonstrated an augmented contractile response only to S6c in isolated rat coronary arteries after organ culture (with or without LPS). These contractile responses by S6c were blocked by the selective ETB receptor antagonist BQ788 in both vessel groups. The augmented contractile response to S6c was supported by immunohistochemistry, where a significant increase in fluorescence intensity for ETB receptors in smooth muscle cells was observed after organ culture. The presence of LPS in the culture medium significantly increased the sensitivity of endothelium-intact coronary artery to S6c as compared to endothelium-denuded segments. Our results showed a significant increase in both ETB receptor protein levels and S6c-induced maximal contraction in coronary arteries upon 24h of organ culture, which was further sensitized by LPS.
Original languageEnglish
JournalVascular Pharmacology
Volume53
Issue number5-6
Pages (from-to)250-7
Number of pages8
ISSN1537-1891
DOIs
Publication statusPublished - 2010

ID: 22524337