MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface. / Listov-Saabye, Nicolai; Jensen, Marianne Blirup; Kiehr, Benedicte; Hansen, Erik Wind; Svendsen, Jette E; Lundby, Anders; Holm, Gitte-Mai Nelander; Oleksiewicz, Martin B.

In: Journal of Applied Toxicology, Vol. 29, No. 6, 2009, p. 470-7.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Listov-Saabye, N, Jensen, MB, Kiehr, B, Hansen, EW, Svendsen, JE, Lundby, A, Holm, G-MN & Oleksiewicz, MB 2009, 'MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface', Journal of Applied Toxicology, vol. 29, no. 6, pp. 470-7. https://doi.org/10.1002/jat.1428

APA

Listov-Saabye, N., Jensen, M. B., Kiehr, B., Hansen, E. W., Svendsen, J. E., Lundby, A., ... Oleksiewicz, M. B. (2009). MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface. Journal of Applied Toxicology, 29(6), 470-7. https://doi.org/10.1002/jat.1428

Vancouver

Listov-Saabye N, Jensen MB, Kiehr B, Hansen EW, Svendsen JE, Lundby A et al. MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface. Journal of Applied Toxicology. 2009;29(6):470-7. https://doi.org/10.1002/jat.1428

Author

Listov-Saabye, Nicolai ; Jensen, Marianne Blirup ; Kiehr, Benedicte ; Hansen, Erik Wind ; Svendsen, Jette E ; Lundby, Anders ; Holm, Gitte-Mai Nelander ; Oleksiewicz, Martin B. / MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface. In: Journal of Applied Toxicology. 2009 ; Vol. 29, No. 6. pp. 470-7.

Bibtex

@article{7fe193a0ac2911debc73000ea68e967b,
title = "MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface",
abstract = "Human mammary cell lines are extensively used for preclinical safety assessment of insulin analogs. However, it is essentially unknown how mitogenic responses can be optimized in mammary cell-based systems. We developed an insulin mitogenicity assay in MCF-7 human mammary adenocarcinoma cells, under low serum (0.1{\%} FCS) and phenol red-free conditions, with 3H thymidine incorporation as endpoint. Based on EC50 values determined from 10-fold dilution series, beta-estradiol was the most potent mitogen, followed by human IGF-1, human AspB10 insulin and native human insulin. AspB10 insulin was significantly more mitogenic than native insulin, validating the ability of the assay to identify hypermitogenic human insulin analogs. With MCF-7 cells on a collagen IV surface, the ranking of mitogens was maintained, but fold mitogenic responses and dynamic range and steepness of dose-response curves were increased. Also, PI3K pathway activation by insulin was enhanced on a collagen IV surface. This study provided the first determination and ranking of the mitogenic potencies of standard reference compounds in an optimized MCF-7 assay. The optimized MCF-7 assay described here is of relevance for in vitro toxicological testing and carcinogenicity safety assessment of new insulin compounds.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Nicolai Listov-Saabye and Jensen, {Marianne Blirup} and Benedicte Kiehr and Hansen, {Erik Wind} and Svendsen, {Jette E} and Anders Lundby and Holm, {Gitte-Mai Nelander} and Oleksiewicz, {Martin B}",
note = "Copyright 2009 John Wiley & Sons, Ltd.",
year = "2009",
doi = "10.1002/jat.1428",
language = "English",
volume = "29",
pages = "470--7",
journal = "Journal of Applied Toxicology",
issn = "0260-437X",
publisher = "JohnWiley & Sons Ltd",
number = "6",

}

RIS

TY - JOUR

T1 - MCF-7 human mammary adenocarcinoma cells exhibit augmented responses to human insulin on a collagen IV surface

AU - Listov-Saabye, Nicolai

AU - Jensen, Marianne Blirup

AU - Kiehr, Benedicte

AU - Hansen, Erik Wind

AU - Svendsen, Jette E

AU - Lundby, Anders

AU - Holm, Gitte-Mai Nelander

AU - Oleksiewicz, Martin B

N1 - Copyright 2009 John Wiley & Sons, Ltd.

PY - 2009

Y1 - 2009

N2 - Human mammary cell lines are extensively used for preclinical safety assessment of insulin analogs. However, it is essentially unknown how mitogenic responses can be optimized in mammary cell-based systems. We developed an insulin mitogenicity assay in MCF-7 human mammary adenocarcinoma cells, under low serum (0.1% FCS) and phenol red-free conditions, with 3H thymidine incorporation as endpoint. Based on EC50 values determined from 10-fold dilution series, beta-estradiol was the most potent mitogen, followed by human IGF-1, human AspB10 insulin and native human insulin. AspB10 insulin was significantly more mitogenic than native insulin, validating the ability of the assay to identify hypermitogenic human insulin analogs. With MCF-7 cells on a collagen IV surface, the ranking of mitogens was maintained, but fold mitogenic responses and dynamic range and steepness of dose-response curves were increased. Also, PI3K pathway activation by insulin was enhanced on a collagen IV surface. This study provided the first determination and ranking of the mitogenic potencies of standard reference compounds in an optimized MCF-7 assay. The optimized MCF-7 assay described here is of relevance for in vitro toxicological testing and carcinogenicity safety assessment of new insulin compounds.

AB - Human mammary cell lines are extensively used for preclinical safety assessment of insulin analogs. However, it is essentially unknown how mitogenic responses can be optimized in mammary cell-based systems. We developed an insulin mitogenicity assay in MCF-7 human mammary adenocarcinoma cells, under low serum (0.1% FCS) and phenol red-free conditions, with 3H thymidine incorporation as endpoint. Based on EC50 values determined from 10-fold dilution series, beta-estradiol was the most potent mitogen, followed by human IGF-1, human AspB10 insulin and native human insulin. AspB10 insulin was significantly more mitogenic than native insulin, validating the ability of the assay to identify hypermitogenic human insulin analogs. With MCF-7 cells on a collagen IV surface, the ranking of mitogens was maintained, but fold mitogenic responses and dynamic range and steepness of dose-response curves were increased. Also, PI3K pathway activation by insulin was enhanced on a collagen IV surface. This study provided the first determination and ranking of the mitogenic potencies of standard reference compounds in an optimized MCF-7 assay. The optimized MCF-7 assay described here is of relevance for in vitro toxicological testing and carcinogenicity safety assessment of new insulin compounds.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1002/jat.1428

DO - 10.1002/jat.1428

M3 - Journal article

C2 - 19338014

VL - 29

SP - 470

EP - 477

JO - Journal of Applied Toxicology

JF - Journal of Applied Toxicology

SN - 0260-437X

IS - 6

ER -

ID: 14774425