Modification of beta-2-microglobulin in sera from patients with small cell lung cancer: evidence for involvement of a serine protease.

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Mogens Holst Nissen, Ole Jannik Bjerrum, T Plesner, M Wilken, M Rørth

Modification of beta-2-microglobulin has been shown to occur in vitro in serum of patients suffering of small cell lung cancer, where it clearly correlated to the clinical course of disease. The cause of serum beta-2-microglobulin modification occurring in these patients is investigated in the present study. This revealed that modification of beta-2-microglobulin is due to cleavage by a serine protease, which is dependent on divalent cations and has a trypsin-like specificity. The process showed the following characteristics: pH optimum at 8.5, heat inactivation by incubation at 56 degrees C for 20 min and temperature optimum at 20 degrees C.
Original languageEnglish
JournalClinical and Experimental Immunology
Volume67
Issue number2
Pages (from-to)425-32
Number of pages7
ISSN0009-9104
Publication statusPublished - 1987

Bibliographical note

Keywords: Carcinoma, Small Cell; Endopeptidases; Humans; Hydrogen-Ion Concentration; Immunoelectrophoresis, Two-Dimensional; Lung Neoplasms; Protease Inhibitors; Serine Endopeptidases; Temperature; beta 2-Microglobulin

ID: 8746804