Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen. / Kristensen, Matilde B; Metzdorff, Stine Broeng; Bergström, Anders; Damlund, Dina Silke Malling; Fink, Lisbeth N; Licht, Tine R; Frøkiær, Hanne.

In: Immunity, Inflammation and Disease, Vol. 3, No. 3, 09.2015, p. 309-320.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kristensen, MB, Metzdorff, SB, Bergström, A, Damlund, DSM, Fink, LN, Licht, TR & Frøkiær, H 2015, 'Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen', Immunity, Inflammation and Disease, vol. 3, no. 3, pp. 309-320. https://doi.org/10.1002/iid3.70

APA

Kristensen, M. B., Metzdorff, S. B., Bergström, A., Damlund, D. S. M., Fink, L. N., Licht, T. R., & Frøkiær, H. (2015). Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen. Immunity, Inflammation and Disease, 3(3), 309-320. https://doi.org/10.1002/iid3.70

Vancouver

Kristensen MB, Metzdorff SB, Bergström A, Damlund DSM, Fink LN, Licht TR et al. Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen. Immunity, Inflammation and Disease. 2015 Sep;3(3):309-320. https://doi.org/10.1002/iid3.70

Author

Kristensen, Matilde B ; Metzdorff, Stine Broeng ; Bergström, Anders ; Damlund, Dina Silke Malling ; Fink, Lisbeth N ; Licht, Tine R ; Frøkiær, Hanne. / Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen. In: Immunity, Inflammation and Disease. 2015 ; Vol. 3, No. 3. pp. 309-320.

Bibtex

@article{3f7b5ff701914f10a65e6fc93bb7ebd6,
title = "Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen",
abstract = "To assess the microbial influence on postnatal hematopoiesis, we examined the role of early life microbial colonization on the composition of leukocyte subsets in the neonatal spleen. A high number of CD11b(+)Gr-1(+) splenocytes present perinatally was sustained for a longer period in conventionally colonized (CONV) mice than in mono-colonized (MC) and germfree (GF) mice, and the CD4(+) T cell population established faster in CONV mice. At the day of birth, compared to GF mice, the expression of Cxcl2 was up-regulated and Arg1 down-regulated in livers of CONV mice. This coincided with lower abundance of polylobed cells in the liver of CONV mice. An earlier peak in the expression of the genes Tjp1, Cdh1, and JamA in intestinal epithelial cells of CONV mice indicated an accelerated closure of the epithelial barrier. In conclusion, we have identified an important microbiota-dependent neonatal hematopoietic event, which we suggest impacts the subsequent development of the T cell population in the murine spleen.",
keywords = "Faculty of Health and Medical Sciences, CD11b+ Gr-1+ myeloid cells, CD4+ T cells, microbiota, neonatal hematopoiesis",
author = "Kristensen, {Matilde B} and Metzdorff, {Stine Broeng} and Anders Bergstr{\"o}m and Damlund, {Dina Silke Malling} and Fink, {Lisbeth N} and Licht, {Tine R} and Hanne Fr{\o}ki{\ae}r",
year = "2015",
month = "9",
doi = "10.1002/iid3.70",
language = "English",
volume = "3",
pages = "309--320",
journal = "Immunity, Inflammation and Disease",
issn = "2050-4527",
publisher = "JohnWiley & Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Neonatal microbial colonization in mice promotes prolonged dominance of CD11b+Gr-1+ cells and accelerated establishment of the CD4+ T cell population in the spleen

AU - Kristensen, Matilde B

AU - Metzdorff, Stine Broeng

AU - Bergström, Anders

AU - Damlund, Dina Silke Malling

AU - Fink, Lisbeth N

AU - Licht, Tine R

AU - Frøkiær, Hanne

PY - 2015/9

Y1 - 2015/9

N2 - To assess the microbial influence on postnatal hematopoiesis, we examined the role of early life microbial colonization on the composition of leukocyte subsets in the neonatal spleen. A high number of CD11b(+)Gr-1(+) splenocytes present perinatally was sustained for a longer period in conventionally colonized (CONV) mice than in mono-colonized (MC) and germfree (GF) mice, and the CD4(+) T cell population established faster in CONV mice. At the day of birth, compared to GF mice, the expression of Cxcl2 was up-regulated and Arg1 down-regulated in livers of CONV mice. This coincided with lower abundance of polylobed cells in the liver of CONV mice. An earlier peak in the expression of the genes Tjp1, Cdh1, and JamA in intestinal epithelial cells of CONV mice indicated an accelerated closure of the epithelial barrier. In conclusion, we have identified an important microbiota-dependent neonatal hematopoietic event, which we suggest impacts the subsequent development of the T cell population in the murine spleen.

AB - To assess the microbial influence on postnatal hematopoiesis, we examined the role of early life microbial colonization on the composition of leukocyte subsets in the neonatal spleen. A high number of CD11b(+)Gr-1(+) splenocytes present perinatally was sustained for a longer period in conventionally colonized (CONV) mice than in mono-colonized (MC) and germfree (GF) mice, and the CD4(+) T cell population established faster in CONV mice. At the day of birth, compared to GF mice, the expression of Cxcl2 was up-regulated and Arg1 down-regulated in livers of CONV mice. This coincided with lower abundance of polylobed cells in the liver of CONV mice. An earlier peak in the expression of the genes Tjp1, Cdh1, and JamA in intestinal epithelial cells of CONV mice indicated an accelerated closure of the epithelial barrier. In conclusion, we have identified an important microbiota-dependent neonatal hematopoietic event, which we suggest impacts the subsequent development of the T cell population in the murine spleen.

KW - Faculty of Health and Medical Sciences

KW - CD11b+ Gr-1+ myeloid cells

KW - CD4+ T cells

KW - microbiota

KW - neonatal hematopoiesis

U2 - 10.1002/iid3.70

DO - 10.1002/iid3.70

M3 - Journal article

VL - 3

SP - 309

EP - 320

JO - Immunity, Inflammation and Disease

JF - Immunity, Inflammation and Disease

SN - 2050-4527

IS - 3

ER -

ID: 144833473