Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump

Research output: Contribution to journalJournal articleResearchpeer-review

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Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump. / Mahmmoud, Yasser Ahmed; Christensen, Søren Brøgger.

In: BBA General Subjects, Vol. 1808, No. 10, 10.2011, p. 2413-2420.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Mahmmoud, YA & Christensen, SB 2011, 'Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump', BBA General Subjects, vol. 1808, no. 10, pp. 2413-2420. https://doi.org/10.1016/j.bbamem.2011.06.025

APA

Mahmmoud, Y. A., & Christensen, S. B. (2011). Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump. BBA General Subjects, 1808(10), 2413-2420. https://doi.org/10.1016/j.bbamem.2011.06.025

Vancouver

Mahmmoud YA, Christensen SB. Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump. BBA General Subjects. 2011 Oct;1808(10):2413-2420. https://doi.org/10.1016/j.bbamem.2011.06.025

Author

Mahmmoud, Yasser Ahmed ; Christensen, Søren Brøgger. / Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump. In: BBA General Subjects. 2011 ; Vol. 1808, No. 10. pp. 2413-2420.

Bibtex

@article{e8ac78bb487c451b89924be2d0227b4a,
title = "Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump",
abstract = "Nigella sativa seed oil was found to contain a modulator of Na,K-ATPase. Separation analyses combined with (1)H NMR and GCMS identified the inhibitory fraction as a mixture of oleic and linoleic acids. These two fatty acids are specifically concentrated in several medicinal plant oils, and have particularly been implicated in decreasing high blood pressure. The ouabain binding site on Na,K-ATPase has also been implicated in blood pressure regulation. Thus, we aimed to determine how these two molecules modify pig kidney Na,K-ATPase. Oleic and linoleic acids did not modify reactions involving the E(1) (Na(+)) conformations of the Na,K-ATPase. In contrast, K(+) dependent reactions were strongly modified after treatment. Oleic and linoleic acids were found to stabilize a pump conformation that binds ouabain with high affinity, i.e., an ion free E(2)P form. Time-resolved binding assays using anthroylouabain, a fluorescent ouabain analog, revealed that the increased ouabain affinity is unique to oleic and linoleic acids, as compared with ¿-linolenic acid, which decreased pump-mediated ATP hydrolysis but did not equally increase ouabain interaction with the pump. Thus, the dynamic changes in plasma levels of oleic and linoleic acids are important in the modulation of the sensitivity of the sodium pump to cardiac glycosides. Given the possible involvement of the cardiac glycoside binding site on Na,K-ATPase in the regulation of hypertension, we suggest oleic acid to be a specific chaperon that modulates interaction of cardiac glycosides with the sodium pump.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Mahmmoud, {Yasser Ahmed} and Christensen, {S{\o}ren Br{\o}gger}",
note = "Keywords: Na,K-ATPase; Nigella Sativa; Oleic acid; Linoleic acid; Ouabain affinity; Anthroyl ouabain",
year = "2011",
month = "10",
doi = "10.1016/j.bbamem.2011.06.025",
language = "English",
volume = "1808",
pages = "2413--2420",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump

AU - Mahmmoud, Yasser Ahmed

AU - Christensen, Søren Brøgger

N1 - Keywords: Na,K-ATPase; Nigella Sativa; Oleic acid; Linoleic acid; Ouabain affinity; Anthroyl ouabain

PY - 2011/10

Y1 - 2011/10

N2 - Nigella sativa seed oil was found to contain a modulator of Na,K-ATPase. Separation analyses combined with (1)H NMR and GCMS identified the inhibitory fraction as a mixture of oleic and linoleic acids. These two fatty acids are specifically concentrated in several medicinal plant oils, and have particularly been implicated in decreasing high blood pressure. The ouabain binding site on Na,K-ATPase has also been implicated in blood pressure regulation. Thus, we aimed to determine how these two molecules modify pig kidney Na,K-ATPase. Oleic and linoleic acids did not modify reactions involving the E(1) (Na(+)) conformations of the Na,K-ATPase. In contrast, K(+) dependent reactions were strongly modified after treatment. Oleic and linoleic acids were found to stabilize a pump conformation that binds ouabain with high affinity, i.e., an ion free E(2)P form. Time-resolved binding assays using anthroylouabain, a fluorescent ouabain analog, revealed that the increased ouabain affinity is unique to oleic and linoleic acids, as compared with ¿-linolenic acid, which decreased pump-mediated ATP hydrolysis but did not equally increase ouabain interaction with the pump. Thus, the dynamic changes in plasma levels of oleic and linoleic acids are important in the modulation of the sensitivity of the sodium pump to cardiac glycosides. Given the possible involvement of the cardiac glycoside binding site on Na,K-ATPase in the regulation of hypertension, we suggest oleic acid to be a specific chaperon that modulates interaction of cardiac glycosides with the sodium pump.

AB - Nigella sativa seed oil was found to contain a modulator of Na,K-ATPase. Separation analyses combined with (1)H NMR and GCMS identified the inhibitory fraction as a mixture of oleic and linoleic acids. These two fatty acids are specifically concentrated in several medicinal plant oils, and have particularly been implicated in decreasing high blood pressure. The ouabain binding site on Na,K-ATPase has also been implicated in blood pressure regulation. Thus, we aimed to determine how these two molecules modify pig kidney Na,K-ATPase. Oleic and linoleic acids did not modify reactions involving the E(1) (Na(+)) conformations of the Na,K-ATPase. In contrast, K(+) dependent reactions were strongly modified after treatment. Oleic and linoleic acids were found to stabilize a pump conformation that binds ouabain with high affinity, i.e., an ion free E(2)P form. Time-resolved binding assays using anthroylouabain, a fluorescent ouabain analog, revealed that the increased ouabain affinity is unique to oleic and linoleic acids, as compared with ¿-linolenic acid, which decreased pump-mediated ATP hydrolysis but did not equally increase ouabain interaction with the pump. Thus, the dynamic changes in plasma levels of oleic and linoleic acids are important in the modulation of the sensitivity of the sodium pump to cardiac glycosides. Given the possible involvement of the cardiac glycoside binding site on Na,K-ATPase in the regulation of hypertension, we suggest oleic acid to be a specific chaperon that modulates interaction of cardiac glycosides with the sodium pump.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.bbamem.2011.06.025

DO - 10.1016/j.bbamem.2011.06.025

M3 - Journal article

C2 - 21767529

VL - 1808

SP - 2413

EP - 2420

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 10

ER -

ID: 37432790