Organ culture of C57BL/6 mouse arteries with LPS as an in vitro model of vascular inflammation

Research output: Contribution to conferencePosterResearch

Emilie Middelbo Outzen, Rahila Mehryar, Harrie C.M. Boonen, Anette Sams Nielsen, Majid Sheykhzade

Background: Vascular inflammation is believed to be involved in the pathogenesis of various cardiovascular diseases, the study of which often involves use of the mouse strain C57BL/6. In vivo studies can, however, be difficult to control and interpret.

Aim of the study: To set up and characterise an in vitro model for studying vascular inflammation and function in cultured arteries from C57BL/6 mice.
Methods: Segments of abdominal aorta and mesenteric arteries (MA) were incubated for 24 hours at 37̊C and 95% O2/5% CO2 in DMEM ± 100 ng/mL LPS. Aorta segments were frozen for molecular studies of inflammatory markers. MA segments were mounted in tissue baths for measurement of isometric contractile force. Functional studies of MA included characterisation of length-tension relationship and pharmacological reactivity to selected agonists.

Results and discussion: Maximum active wall tension of C57BL/6 MA was achieved at a normalisation factor of 0.9 (0.91 ± 0.06, mean ± SEM, n = 9) as observed (0.85 ± 0.06, mean ± SEM, n = 3) and previously described in rat MA (Mulvany and Halpern, 1977). Furthermore, preliminary findings show that organ culture with 100 ng/mL LPS decreases endothelium-dependent dilation of C57BL/6 MA along with increased expression of inflammatory markers in aorta.

Conclusions: In C57BL/6 MA, maximum active wall tension was achieved with a normalisation factor of 0.9. Furthermore, organ culture with LPS induces vascular inflammation and functional changes in C57BL/6 arteries. The in vitro model allows further assessment of relevant vasoactive systems and studies of possible protective mediators.
Original languageEnglish
Publication date28 Aug 2013
Number of pages1
Publication statusPublished - 28 Aug 2013
EventDRA Summer School 2013 - Hotel Scandic, Copenhagen, Denmark
Duration: 28 Aug 201329 Aug 2013

Seminar

SeminarDRA Summer School 2013
LocationHotel Scandic
CountryDenmark
CityCopenhagen
Period28/08/201329/08/2013

ID: 49753101