P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently

Research output: Contribution to journalJournal articleResearchpeer-review

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P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently. / Klærke, Susanne Edeling Hede; Amstrup, Jan; Klærke, Dan Arne; Novak, Ivana.

In: Pflügers Archiv - European Journal of Physiology, Vol. 450, No. 6, 2005, p. 429-436.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Klærke, SEH, Amstrup, J, Klærke, DA & Novak, I 2005, 'P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently', Pflügers Archiv - European Journal of Physiology, vol. 450, no. 6, pp. 429-436. https://doi.org/10.1007/s00424-005-1433-3

APA

Klærke, S. E. H., Amstrup, J., Klærke, D. A., & Novak, I. (2005). P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently. Pflügers Archiv - European Journal of Physiology, 450(6), 429-436. https://doi.org/10.1007/s00424-005-1433-3

Vancouver

Klærke SEH, Amstrup J, Klærke DA, Novak I. P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently. Pflügers Archiv - European Journal of Physiology. 2005;450(6):429-436. https://doi.org/10.1007/s00424-005-1433-3

Author

Klærke, Susanne Edeling Hede ; Amstrup, Jan ; Klærke, Dan Arne ; Novak, Ivana. / P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently. In: Pflügers Archiv - European Journal of Physiology. 2005 ; Vol. 450, No. 6. pp. 429-436.

Bibtex

@article{f41f4b60a1bf11ddb6ae000ea68e967b,
title = "P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently",
abstract = "Extracellular ATP is an important regulator of transepithelial transport in a number of tissues. In pancreatic ducts, we have shown that ATP modulates epithelial K+ channels via purinergic receptors, most likely the P2Y2 and P2Y4 receptors, but the identity of the involved K+ channels was not clear. In this study, we show by RT-PCR analysis that rat pancreatic ducts express Ca(2+)-activated K+ channels of intermediate conductance (IK) and big conductance (BK), but not small conductance (SK). Possible interactions between P2Y receptors and these Ca(2+)-activated K+ channels were examined in co-expression experiments in Xenopus laevis oocytes. K+ channel activity was measured electrophysiologically in oocytes stimulated with UTP (0.1 mM). UTP stimulation of oocytes expressing P2Y4 receptors and BK channels resulted in a 30{\%} increase in the current through the expressed channels. In contrast, stimulation of P2Y2 receptors led to a 20{\%} inhibition of co-expressed BK channel activity, a response that was sensitive to TEA. Furthermore, co-expression of IK channels with P2Y4 and P2Y2 receptors resulted in a large hyperpolarization and 22-fold and 5-fold activ ation of currents by UTP, respectively. Taken together, this study shows that there are different interactions between the subtypes of P2Y purinergic receptors and different Ca(2+)-activated K+ channels.",
keywords = "Former LIFE faculty, Maxi K, KCNMA1, SK4, KCNN4, Pancreas, Purinergic receptors, Ducts, Slo",
author = "Kl{\ae}rke, {Susanne Edeling Hede} and Jan Amstrup and Kl{\ae}rke, {Dan Arne} and Ivana Novak",
note = "Keywords: Animals; Female; Intermediate-Conductance Calcium-Activated Potassium Channels; Large-Conductance Calcium-Activated Potassium Channels; Oocytes; Pancreatic Ducts; Potassium Channels, Calcium-Activated; Rats; Rats, Wistar; Receptors, Purinergic P2; Reverse Transcriptase Polymerase Chain Reaction; Small-Conductance Calcium-Activated Potassium Channels; Uridine Triphosphate; Xenopus laevis",
year = "2005",
doi = "10.1007/s00424-005-1433-3",
language = "English",
volume = "450",
pages = "429--436",
journal = "Pfl{\"u}gers Archiv - European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - P2Y2 and P2Y4 receptors regulate pancreatic Ca²+-activated K+ channels differently

AU - Klærke, Susanne Edeling Hede

AU - Amstrup, Jan

AU - Klærke, Dan Arne

AU - Novak, Ivana

N1 - Keywords: Animals; Female; Intermediate-Conductance Calcium-Activated Potassium Channels; Large-Conductance Calcium-Activated Potassium Channels; Oocytes; Pancreatic Ducts; Potassium Channels, Calcium-Activated; Rats; Rats, Wistar; Receptors, Purinergic P2; Reverse Transcriptase Polymerase Chain Reaction; Small-Conductance Calcium-Activated Potassium Channels; Uridine Triphosphate; Xenopus laevis

PY - 2005

Y1 - 2005

N2 - Extracellular ATP is an important regulator of transepithelial transport in a number of tissues. In pancreatic ducts, we have shown that ATP modulates epithelial K+ channels via purinergic receptors, most likely the P2Y2 and P2Y4 receptors, but the identity of the involved K+ channels was not clear. In this study, we show by RT-PCR analysis that rat pancreatic ducts express Ca(2+)-activated K+ channels of intermediate conductance (IK) and big conductance (BK), but not small conductance (SK). Possible interactions between P2Y receptors and these Ca(2+)-activated K+ channels were examined in co-expression experiments in Xenopus laevis oocytes. K+ channel activity was measured electrophysiologically in oocytes stimulated with UTP (0.1 mM). UTP stimulation of oocytes expressing P2Y4 receptors and BK channels resulted in a 30% increase in the current through the expressed channels. In contrast, stimulation of P2Y2 receptors led to a 20% inhibition of co-expressed BK channel activity, a response that was sensitive to TEA. Furthermore, co-expression of IK channels with P2Y4 and P2Y2 receptors resulted in a large hyperpolarization and 22-fold and 5-fold activ ation of currents by UTP, respectively. Taken together, this study shows that there are different interactions between the subtypes of P2Y purinergic receptors and different Ca(2+)-activated K+ channels.

AB - Extracellular ATP is an important regulator of transepithelial transport in a number of tissues. In pancreatic ducts, we have shown that ATP modulates epithelial K+ channels via purinergic receptors, most likely the P2Y2 and P2Y4 receptors, but the identity of the involved K+ channels was not clear. In this study, we show by RT-PCR analysis that rat pancreatic ducts express Ca(2+)-activated K+ channels of intermediate conductance (IK) and big conductance (BK), but not small conductance (SK). Possible interactions between P2Y receptors and these Ca(2+)-activated K+ channels were examined in co-expression experiments in Xenopus laevis oocytes. K+ channel activity was measured electrophysiologically in oocytes stimulated with UTP (0.1 mM). UTP stimulation of oocytes expressing P2Y4 receptors and BK channels resulted in a 30% increase in the current through the expressed channels. In contrast, stimulation of P2Y2 receptors led to a 20% inhibition of co-expressed BK channel activity, a response that was sensitive to TEA. Furthermore, co-expression of IK channels with P2Y4 and P2Y2 receptors resulted in a large hyperpolarization and 22-fold and 5-fold activ ation of currents by UTP, respectively. Taken together, this study shows that there are different interactions between the subtypes of P2Y purinergic receptors and different Ca(2+)-activated K+ channels.

KW - Former LIFE faculty

KW - Maxi K

KW - KCNMA1

KW - SK4

KW - KCNN4

KW - Pancreas

KW - Purinergic receptors

KW - Ducts

KW - Slo

U2 - 10.1007/s00424-005-1433-3

DO - 10.1007/s00424-005-1433-3

M3 - Journal article

VL - 450

SP - 429

EP - 436

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

SN - 0031-6768

IS - 6

ER -

ID: 7998970