Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells

Research output: Contribution to journalJournal articleResearchpeer-review

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Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells. / Moreno Delgado, David; Møller, Thor C; Ster, Jeanne; Giraldo, Jesús; Maurel, Damien; Rovira, Xavier; Scholler, Pauline; Zwier, Jurriaan M; Perroy, Julie; Durroux, Thierry; Trinquet, Eric; Prezeau, Laurent; Rondard, Philippe; Pin, Jean-Philippe.

In: eLife, Vol. 6, 29.06.2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Moreno Delgado, D, Møller, TC, Ster, J, Giraldo, J, Maurel, D, Rovira, X, Scholler, P, Zwier, JM, Perroy, J, Durroux, T, Trinquet, E, Prezeau, L, Rondard, P & Pin, J-P 2017, 'Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells', eLife, vol. 6. https://doi.org/10.7554/eLife.25233

APA

Moreno Delgado, D., Møller, T. C., Ster, J., Giraldo, J., Maurel, D., Rovira, X., ... Pin, J-P. (2017). Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells. eLife, 6. https://doi.org/10.7554/eLife.25233

Vancouver

Moreno Delgado D, Møller TC, Ster J, Giraldo J, Maurel D, Rovira X et al. Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells. eLife. 2017 Jun 29;6. https://doi.org/10.7554/eLife.25233

Author

Moreno Delgado, David ; Møller, Thor C ; Ster, Jeanne ; Giraldo, Jesús ; Maurel, Damien ; Rovira, Xavier ; Scholler, Pauline ; Zwier, Jurriaan M ; Perroy, Julie ; Durroux, Thierry ; Trinquet, Eric ; Prezeau, Laurent ; Rondard, Philippe ; Pin, Jean-Philippe. / Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells. In: eLife. 2017 ; Vol. 6.

Bibtex

@article{98fa58afc57d4e7fb0bf2acca99f6bcb,
title = "Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells",
abstract = "Metabotropic glutamate receptors (mGluRs) are mandatory dimers playing important roles in regulating CNS function. Although assumed to form exclusive homodimers, 16 possible heterodimeric mGluRs have been proposed but their existence in native cells remains elusive. Here, we set up two assays to specifically identify the pharmacological properties of rat mGlu heterodimers composed of mGlu2 and 4 subunits. We used either a heterodimer-specific conformational LRET-based biosensor or a system that guarantees the cell surface targeting of the heterodimer only. We identified mGlu2-4 specific pharmacological fingerprints that were also observed in a neuronal cell line and in lateral perforant path terminals naturally expressing mGlu2 and mGlu4. These results bring strong evidence for the existence of mGlu2-4 heterodimers in native cells. In addition to reporting a general approach to characterize heterodimeric mGluRs, our study opens new avenues to understanding the pathophysiological roles of mGlu heterodimers.",
keywords = "Animals, Bridged Bicyclo Compounds/pharmacology, Cells, Cultured, Embryo, Mammalian/cytology, Excitatory Amino Acid Agonists/pharmacology, HEK293 Cells, Hippocampus/cytology, Humans, Neurons/cytology, Protein Multimerization/drug effects, Rats, Rats, Sprague-Dawley, Receptors, Metabotropic Glutamate/chemistry",
author = "{Moreno Delgado}, David and M{\o}ller, {Thor C} and Jeanne Ster and Jes{\'u}s Giraldo and Damien Maurel and Xavier Rovira and Pauline Scholler and Zwier, {Jurriaan M} and Julie Perroy and Thierry Durroux and Eric Trinquet and Laurent Prezeau and Philippe Rondard and Jean-Philippe Pin",
year = "2017",
month = "6",
day = "29",
doi = "10.7554/eLife.25233",
language = "English",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells

AU - Moreno Delgado, David

AU - Møller, Thor C

AU - Ster, Jeanne

AU - Giraldo, Jesús

AU - Maurel, Damien

AU - Rovira, Xavier

AU - Scholler, Pauline

AU - Zwier, Jurriaan M

AU - Perroy, Julie

AU - Durroux, Thierry

AU - Trinquet, Eric

AU - Prezeau, Laurent

AU - Rondard, Philippe

AU - Pin, Jean-Philippe

PY - 2017/6/29

Y1 - 2017/6/29

N2 - Metabotropic glutamate receptors (mGluRs) are mandatory dimers playing important roles in regulating CNS function. Although assumed to form exclusive homodimers, 16 possible heterodimeric mGluRs have been proposed but their existence in native cells remains elusive. Here, we set up two assays to specifically identify the pharmacological properties of rat mGlu heterodimers composed of mGlu2 and 4 subunits. We used either a heterodimer-specific conformational LRET-based biosensor or a system that guarantees the cell surface targeting of the heterodimer only. We identified mGlu2-4 specific pharmacological fingerprints that were also observed in a neuronal cell line and in lateral perforant path terminals naturally expressing mGlu2 and mGlu4. These results bring strong evidence for the existence of mGlu2-4 heterodimers in native cells. In addition to reporting a general approach to characterize heterodimeric mGluRs, our study opens new avenues to understanding the pathophysiological roles of mGlu heterodimers.

AB - Metabotropic glutamate receptors (mGluRs) are mandatory dimers playing important roles in regulating CNS function. Although assumed to form exclusive homodimers, 16 possible heterodimeric mGluRs have been proposed but their existence in native cells remains elusive. Here, we set up two assays to specifically identify the pharmacological properties of rat mGlu heterodimers composed of mGlu2 and 4 subunits. We used either a heterodimer-specific conformational LRET-based biosensor or a system that guarantees the cell surface targeting of the heterodimer only. We identified mGlu2-4 specific pharmacological fingerprints that were also observed in a neuronal cell line and in lateral perforant path terminals naturally expressing mGlu2 and mGlu4. These results bring strong evidence for the existence of mGlu2-4 heterodimers in native cells. In addition to reporting a general approach to characterize heterodimeric mGluRs, our study opens new avenues to understanding the pathophysiological roles of mGlu heterodimers.

KW - Animals

KW - Bridged Bicyclo Compounds/pharmacology

KW - Cells, Cultured

KW - Embryo, Mammalian/cytology

KW - Excitatory Amino Acid Agonists/pharmacology

KW - HEK293 Cells

KW - Hippocampus/cytology

KW - Humans

KW - Neurons/cytology

KW - Protein Multimerization/drug effects

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptors, Metabotropic Glutamate/chemistry

U2 - 10.7554/eLife.25233

DO - 10.7554/eLife.25233

M3 - Journal article

VL - 6

JO - eLife

JF - eLife

SN - 2050-084X

ER -

ID: 200178407