Polyamines in spermatocytes and residual bodies of rat testis

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Masashi Shin, Lars-Inge Larsson, Kunio Fujiwara

Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and proppidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.

Original languageEnglish
JournalHistochemistry and Cell Biology
Volume127
Issue number6
Pages (from-to)649-655
Number of pages7
ISSN0948-6143
DOIs
Publication statusPublished - 2007

    Research areas

  • LIFE - Polyamines, Immunocytochemistry, Residual body, Double immunofluorescence, Rat

ID: 8078480