Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

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Cathrine Bie Petersen, Ann-Britt Nygaard Hillig, Birgitte Viuff, Merete Fredholm, Bent Aasted, Jan Salomonsen

Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

Original languageEnglish
JournalDevelopmental & Comparative Immunology
Volume31
Issue number6
Pages (from-to)618-631
Number of pages14
ISSN0145-305X
DOIs
Publication statusPublished - 2007

    Research areas

  • LIFE - SWC9, NPP1, Swine, Macrophage

ID: 8043334