Primary structure and conformational analysis of peptide methionine-tyrosine, a peptide related to neuropeptide Y and peptide YY isolated from lamprey intestine

Research output: Contribution to journalJournal articleResearchpeer-review

J M Conlon, B Bjørnholm, Flemming Steen Jørgensen, J H Youson, T W Schwartz

A peptide belonging to the pancreatic-polypeptide-fold family of regulatory peptides has been isolated from the intestine of an Agnathan, the sea lamprey (Petromyzon marinus). The primary structure of the peptide (termed peptide methionine-tyrosine) was established as Met-Pro-Pro-Lys-Pro-Asp-Asn- Pro-Ser-Pro10-Asp-Ala-Ser-Pro-Glu-Leu-Ser-Lys-Tyr20-Met-Leu- Ala-Val-Arg-Asn- Tyr-Ile-Asn-Leu30-Ile-Thr-Arg-Gln-Arg-Tyr CONH2. This sequence shows stronger structural similarity with pig neuropeptide Y (64%), particularly in the COOH-terminal region, than with pig peptide tyrosine--tyrosine (61%) or with pig pancreatic polypeptide (42%). Molecular modelling and dynamic simulation, based upon sequence similarity with turkey pancreatic polypeptide, indicates that the conformations of the polyproline-helix-like region (residues 1-8) and the alpha-helical region (residues 15-30) in turkey pancreatic polypeptide are conserved in peptide methionine-tyrosine, and that non-bonded interactions between these domains have preserved the overall polypeptide fold in the molecule. The substitution of the otherwise totally conserved Gly9 residue by serine in lamprey peptide methionine-tyrosine, however, results in a preferred structure in which the conformation of the beta-turn between the two helical domains (residues 9-14) is appreciably different.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume199
Issue number2
Pages (from-to)293-8
Number of pages6
ISSN0014-2956
DOIs
Publication statusPublished - 1991

    Research areas

  • Amino Acid Sequence, Animals, Chromatography, Gel, Gastrointestinal Hormones, Intestine, Small, Lampreys, Models, Molecular, Molecular Sequence Data, Neuropeptide Y, Peptide YY, Peptides, Protein Conformation, Sequence Homology, Nucleic Acid

ID: 38394633