Regulatory T cells in human immunodeficiency virus-infected patients are elevated and independent of immunological and virological status, as well as initiation of highly active anti-retroviral therapy

Research output: Contribution to journalJournal articleResearchpeer-review

  • J.C. Gaardbo
  • S.D. Nielsen
  • S.J. Vedel
  • Annette Kjær Ersbøll
  • Lene Holm Harritshøj
  • Lars P. Ryder
  • J.O. Nielsen
  • J. Kolte
  • J C Gaardbo
  • Susanne Dam Nielsen
  • Signe Juul Vedel
  • A K Ersbøll
  • L Harritshøj
  • L P Ryder
  • Jens Ole Nielsen
  • Lilian Kolte
Infection with human immunodeficiency virus (HIV) causes a dysregulation of the immune system. This is caused by HIV-specific as well as non-specific mechanisms and has not been explained fully. In particular, knowledge is lacking about the potential role of host-mediated immunosuppressive mechanisms. During recent years it has become evident that a subpopulation of T cells [T regulatory (T(regs))] play a major role in sustaining tolerance to self-antigens. To investigate the influence of initiation of highly active anti-retroviral therapy (HAART) on the T(reg) level in HIV-infected patients we have conducted a prospective study enrolling treatment-naive HIV-infected patients just prior to starting treatment with HAART, measuring levels of T(regs) by flow cytometry and mRNA expression of forkhead box P3 (FoxP3) at weeks 0, 4, 12 and 24 of treatment. In this prospective study neither the percentage of CD4(+)CD25(high+) nor the expression of FoxP3 changed significantly during 24 weeks of HAART. Furthermore, HIV patients have higher T(regs) measured as percentages of CD4(+)CD25(high+) cells paralleled by higher levels of FoxP3 compared with healthy controls. The elevated level of T(regs) was found to be independent of both immunological and virological status, indicating that initiation of HAART has minor effects on the T(reg) level in HIV-infected patients.
Original languageEnglish
JournalClinical and Experimental Immunology
Volume154
Issue number1
Pages (from-to)80-6
Number of pages6
ISSN0009-9104
DOIs
Publication statusPublished - 2008

    Research areas

  • Former LIFE faculty - flow cytometry/FACS, highly active anti-retroviral therapy, HIV, immune regulation

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