Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells

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Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells. / Juel, Helene Bæk; Kaestel, Charlotte; Folkersen, Lasse; Faber, Carsten; Heegaard, Niels Henrik Helweg; Borup, Rehannah ; Nissen, Mogens Holst.

In: Experimental Eye Research, Vol. 92, No. 3, 2011, p. 180-88.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Juel, HB, Kaestel, C, Folkersen, L, Faber, C, Heegaard, NHH, Borup, R & Nissen, MH 2011, 'Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells', Experimental Eye Research, vol. 92, no. 3, pp. 180-88. https://doi.org/10.1016/j.exer.2011.01.003

APA

Juel, H. B., Kaestel, C., Folkersen, L., Faber, C., Heegaard, N. H. H., Borup, R., & Nissen, M. H. (2011). Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells. Experimental Eye Research, 92(3), 180-88. https://doi.org/10.1016/j.exer.2011.01.003

Vancouver

Juel HB, Kaestel C, Folkersen L, Faber C, Heegaard NHH, Borup R et al. Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells. Experimental Eye Research. 2011;92(3):180-88. https://doi.org/10.1016/j.exer.2011.01.003

Author

Juel, Helene Bæk ; Kaestel, Charlotte ; Folkersen, Lasse ; Faber, Carsten ; Heegaard, Niels Henrik Helweg ; Borup, Rehannah ; Nissen, Mogens Holst. / Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells. In: Experimental Eye Research. 2011 ; Vol. 92, No. 3. pp. 180-88.

Bibtex

@article{dc5698d78ae84ce380b0f2aaecd45798,
title = "Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells",
abstract = "In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------",
keywords = "Faculty of Health and Medical Sciences, RPE, komplement, T celler",
author = "Juel, {Helene B{\ae}k} and Charlotte Kaestel and Lasse Folkersen and Carsten Faber and Heegaard, {Niels Henrik Helweg} and Rehannah Borup and Nissen, {Mogens Holst}",
year = "2011",
doi = "10.1016/j.exer.2011.01.003",
language = "English",
volume = "92",
pages = "180--88",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press",
number = "3",

}

RIS

TY - JOUR

T1 - Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells

AU - Juel, Helene Bæk

AU - Kaestel, Charlotte

AU - Folkersen, Lasse

AU - Faber, Carsten

AU - Heegaard, Niels Henrik Helweg

AU - Borup, Rehannah

AU - Nissen, Mogens Holst

PY - 2011

Y1 - 2011

N2 - In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------

AB - In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------

KW - Faculty of Health and Medical Sciences

KW - RPE

KW - komplement

KW - T celler

U2 - 10.1016/j.exer.2011.01.003

DO - 10.1016/j.exer.2011.01.003

M3 - Journal article

VL - 92

SP - 180

EP - 188

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 3

ER -

ID: 38304547