Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate

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Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate. / Kolko, Miriam; Nielsen, Marianne; Bazan, Nicolas G; Diemer, Nils H; Kolko, Miriam.

In: Journal of Neuroscience Research, Vol. 69, No. 2, 15.07.2002, p. 169-77.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kolko, M, Nielsen, M, Bazan, NG, Diemer, NH & Kolko, M 2002, 'Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate', Journal of Neuroscience Research, vol. 69, no. 2, pp. 169-77. https://doi.org/10.1002/jnr.10288

APA

Kolko, M., Nielsen, M., Bazan, N. G., Diemer, N. H., & Kolko, M. (2002). Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate. Journal of Neuroscience Research, 69(2), 169-77. https://doi.org/10.1002/jnr.10288

Vancouver

Kolko M, Nielsen M, Bazan NG, Diemer NH, Kolko M. Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate. Journal of Neuroscience Research. 2002 Jul 15;69(2):169-77. https://doi.org/10.1002/jnr.10288

Author

Kolko, Miriam ; Nielsen, Marianne ; Bazan, Nicolas G ; Diemer, Nils H ; Kolko, Miriam. / Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate. In: Journal of Neuroscience Research. 2002 ; Vol. 69, No. 2. pp. 169-77.

Bibtex

@article{775d65fce63d454cae5c8b8c66788847,
title = "Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate",
abstract = "Agonists of the binding site for secretory phospholipase A(2) (sPLA(2)) potentiate glutamate-induced neuronal cell death in primary cell cultures and in vivo (Kolko et al. [1996] J. Biol. Chem. 271:32722; Kolko et al. [1999] Neurosci. Lett. 274:167]. Here, we tested the hypothesis that COX-2 expression participates in the brain response to sPLA(2). sPLA(2)-OS(2), a selective ligand of a neuronal sPLA(2)-binding site, was injected into the rat striatum, and early-response gene expression was monitored by in situ hybridization using (35)S-radiolabeled oligonucleotide probes and immunohistochemistry. An up-regulation of COX-2, c-fos, and c-jun, but not COX-1, was observed around the lesion as well as in the neocortex 4 hr after the injection. Hippocampal up-regulation of COX-2 was seen in dentate gyrus 8 hr after injection. When glutamate was injected, up-regulation of the early-response genes peaked after 2 hr. Our studies showed 1) that sPLA(2) selectively induced neuronal COX-2; 2) that this induction was delayed (4 hr after injection of sPLA(2)) compared with that elicited by glutamate (2 hr after injection), suggesting different signaling; and 3) that c-fos and c-jun were induced around the infarct area as soon as 2 hr after injection, but in other aspects followed a time course similar to that of COX-2. We conclude that sPLA(2) may modulate neuronal COX-2 expression through mechanisms that differ from those of glutamate-induced COX-2 expression.",
keywords = "Animals, Corpus Striatum, Cyclooxygenase 1, Cyclooxygenase 2, Genes, fos, Genes, jun, Glutamic Acid, Immunohistochemistry, In Situ Hybridization, Isoenzymes, Male, Membrane Proteins, Neurons, Phospholipases A, Prostaglandin-Endoperoxide Synthases, Rats, Rats, Wistar, Time Factors, Up-Regulation",
author = "Miriam Kolko and Marianne Nielsen and Bazan, {Nicolas G} and Diemer, {Nils H} and Miriam Kolko",
note = "Copyright 2002 Wiley-Liss, Inc.",
year = "2002",
month = "7",
day = "15",
doi = "10.1002/jnr.10288",
language = "English",
volume = "69",
pages = "169--77",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "JohnWiley & Sons, Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Secretory phospholipase A(2) induces delayed neuronal COX-2 expression compared with glutamate

AU - Kolko, Miriam

AU - Nielsen, Marianne

AU - Bazan, Nicolas G

AU - Diemer, Nils H

AU - Kolko, Miriam

N1 - Copyright 2002 Wiley-Liss, Inc.

PY - 2002/7/15

Y1 - 2002/7/15

N2 - Agonists of the binding site for secretory phospholipase A(2) (sPLA(2)) potentiate glutamate-induced neuronal cell death in primary cell cultures and in vivo (Kolko et al. [1996] J. Biol. Chem. 271:32722; Kolko et al. [1999] Neurosci. Lett. 274:167]. Here, we tested the hypothesis that COX-2 expression participates in the brain response to sPLA(2). sPLA(2)-OS(2), a selective ligand of a neuronal sPLA(2)-binding site, was injected into the rat striatum, and early-response gene expression was monitored by in situ hybridization using (35)S-radiolabeled oligonucleotide probes and immunohistochemistry. An up-regulation of COX-2, c-fos, and c-jun, but not COX-1, was observed around the lesion as well as in the neocortex 4 hr after the injection. Hippocampal up-regulation of COX-2 was seen in dentate gyrus 8 hr after injection. When glutamate was injected, up-regulation of the early-response genes peaked after 2 hr. Our studies showed 1) that sPLA(2) selectively induced neuronal COX-2; 2) that this induction was delayed (4 hr after injection of sPLA(2)) compared with that elicited by glutamate (2 hr after injection), suggesting different signaling; and 3) that c-fos and c-jun were induced around the infarct area as soon as 2 hr after injection, but in other aspects followed a time course similar to that of COX-2. We conclude that sPLA(2) may modulate neuronal COX-2 expression through mechanisms that differ from those of glutamate-induced COX-2 expression.

AB - Agonists of the binding site for secretory phospholipase A(2) (sPLA(2)) potentiate glutamate-induced neuronal cell death in primary cell cultures and in vivo (Kolko et al. [1996] J. Biol. Chem. 271:32722; Kolko et al. [1999] Neurosci. Lett. 274:167]. Here, we tested the hypothesis that COX-2 expression participates in the brain response to sPLA(2). sPLA(2)-OS(2), a selective ligand of a neuronal sPLA(2)-binding site, was injected into the rat striatum, and early-response gene expression was monitored by in situ hybridization using (35)S-radiolabeled oligonucleotide probes and immunohistochemistry. An up-regulation of COX-2, c-fos, and c-jun, but not COX-1, was observed around the lesion as well as in the neocortex 4 hr after the injection. Hippocampal up-regulation of COX-2 was seen in dentate gyrus 8 hr after injection. When glutamate was injected, up-regulation of the early-response genes peaked after 2 hr. Our studies showed 1) that sPLA(2) selectively induced neuronal COX-2; 2) that this induction was delayed (4 hr after injection of sPLA(2)) compared with that elicited by glutamate (2 hr after injection), suggesting different signaling; and 3) that c-fos and c-jun were induced around the infarct area as soon as 2 hr after injection, but in other aspects followed a time course similar to that of COX-2. We conclude that sPLA(2) may modulate neuronal COX-2 expression through mechanisms that differ from those of glutamate-induced COX-2 expression.

KW - Animals

KW - Corpus Striatum

KW - Cyclooxygenase 1

KW - Cyclooxygenase 2

KW - Genes, fos

KW - Genes, jun

KW - Glutamic Acid

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Isoenzymes

KW - Male

KW - Membrane Proteins

KW - Neurons

KW - Phospholipases A

KW - Prostaglandin-Endoperoxide Synthases

KW - Rats

KW - Rats, Wistar

KW - Time Factors

KW - Up-Regulation

U2 - 10.1002/jnr.10288

DO - 10.1002/jnr.10288

M3 - Journal article

VL - 69

SP - 169

EP - 177

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 2

ER -

ID: 128614405