Secretory phospholipase A2-mediated neuronal cell death involves glutamate ionotropic receptors

Research output: Contribution to journalJournal articleResearchpeer-review

Miriam Kolko, Elena B de Turco, Nils Henrik Diemer, Nicolas G Bazan, Miriam Kolko

To define the significance of glutamate ionotropic receptors in sPLA -mediated neuronal cell death we used the NMDA receptor antagonist MK-801 and the AMPA receptor antagonist PNQX. In primary neuronal cell cultures both MK-801 and PNQX inhibited sPLA - and glutamate-induced neuronal death. [ H]Arachidonic acid release induced by both sPLA and glutamate was partially blocked by MK-801, indicating that the glutamate-NMDA-cPLA pathway contributes to sPLA -induced arachidonic acid release. Systemic administration of MK-801 to rats that had sPLA injected into the right striatum significantly decreased neuronal cell death. We conclude that glutamatergic synaptic activity modulates sPLA -induced neuronal cell death.

Original languageEnglish
JournalNeuroReport
Volume13
Issue number15
Pages (from-to)1963-6
Number of pages4
ISSN0959-4965
Publication statusPublished - 28 Oct 2002

    Research areas

  • Animals, Arachidonic Acid, Astrocytes, Body Temperature, Brain, Cell Death, Cells, Cultured, Cerebral Infarction, Dizocilpine Maleate, Dose-Response Relationship, Drug, Excitatory Amino Acid Antagonists, Excitatory Amino Acids, Fetus, Group II Phospholipases A2, Humans, Neostriatum, Neurons, Phospholipases A, Phospholipases A2, Quinoxalines, Rats, Rats, Wistar, Receptors, AMPA, Receptors, N-Methyl-D-Aspartate

ID: 128615058