Serum amyloid A isoforms in serum and synovial fluid from spontaneously diseased dogs with joint diseases or other conditions

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Mads Jens Kjelgaard-Hansen, Michelle B. Christensen, Marcel Huisung Lee, Asger Lundorff Jensen, Stine Jacobsen

Serum amyloid A (SAA) is a major acute phase protein in dogs. However, knowledge of qualitative properties of canine SAA and extent of its synthesis in extrahepatic tissues is limited. The aim of the study was to investigate expression of different SAA isoforms in serum and synovial fluid in samples obtained from dogs (n = 16) suffering from different inflammatory or non-inflammatory conditions, which were either related or unrelated to joints. Expression of SAA isoforms was visualized by denaturing isoelectric focusing and Western blotting. Serum amyloid A was present in serum from all dogs with systemic inflammatory activity, and up to four major isoforms with apparent isoelectric points between 6.1 and 7.9 were identified. In synovial fluid from inflamed joints one or more highly alkaline SAA isoforms (with apparent isoelectric points above 9.3) were identified, with data suggesting local production of these isoforms in the canine inflamed joint.
Original languageEnglish
JournalVeterinary Immunology and Immunopathology
Volume117
Issue number3-4
Pages (from-to)296-301
Number of pages6
ISSN0165-2427
DOIs
Publication statusPublished - 2007

    Research areas

  • LIFE - Acute phase proteins, Arthritis, Canine, Serum amyloid A, Isoforms, Isoelectric focusing

ID: 8098089