Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins. / Laustsen, Andreas Hougaard; Lohse, Brian; Rasmussen, Arne Redsted.

In: Toxicon, Vol. 99, 2015, p. 23-35.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Laustsen, AH, Lohse, B & Rasmussen, AR 2015, 'Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins' Toxicon, vol. 99, pp. 23-35. https://doi.org/10.1016/j.toxicon.2015.03.001

APA

Laustsen, A. H., Lohse, B., & Rasmussen, A. R. (2015). Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins. Toxicon, 99, 23-35. https://doi.org/10.1016/j.toxicon.2015.03.001

Vancouver

Laustsen AH, Lohse B, Rasmussen AR. Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins. Toxicon. 2015;99:23-35. https://doi.org/10.1016/j.toxicon.2015.03.001

Author

Laustsen, Andreas Hougaard ; Lohse, Brian ; Rasmussen, Arne Redsted. / Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins. In: Toxicon. 2015 ; Vol. 99. pp. 23-35.

Bibtex

@article{f1547dc658874d9b84292aaa639c0baf,
title = "Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins",
abstract = "The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5{\%}; including 24.3{\%} cytotoxins and 53.2{\%} neurotoxins) and phospholipases A2 (13.5{\%}). It also contains lower proportions of components belonging to nerve growth factor, ohanin/vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, l-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most relevant lethal components, categorized by means of a ‘toxicity score’, were α-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical α-neurotoxins were obtained. As expected, no neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide prospects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening.",
keywords = "The Faculty of Health and Medical Sciences, Immunity, Human IgG response, Toxicity , Snake venom: proteomics , Monocled cobra , Naja kaouthia",
author = "Laustsen, {Andreas Hougaard} and Brian Lohse and Rasmussen, {Arne Redsted}",
year = "2015",
doi = "10.1016/j.toxicon.2015.03.001",
language = "English",
volume = "99",
pages = "23--35",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins

AU - Laustsen, Andreas Hougaard

AU - Lohse, Brian

AU - Rasmussen, Arne Redsted

PY - 2015

Y1 - 2015

N2 - The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5%; including 24.3% cytotoxins and 53.2% neurotoxins) and phospholipases A2 (13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, l-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most relevant lethal components, categorized by means of a ‘toxicity score’, were α-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical α-neurotoxins were obtained. As expected, no neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide prospects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening.

AB - The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5%; including 24.3% cytotoxins and 53.2% neurotoxins) and phospholipases A2 (13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, l-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most relevant lethal components, categorized by means of a ‘toxicity score’, were α-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical α-neurotoxins were obtained. As expected, no neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide prospects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening.

KW - The Faculty of Health and Medical Sciences

KW - Immunity

KW - Human IgG response

KW - Toxicity

KW - Snake venom: proteomics

KW - Monocled cobra

KW - Naja kaouthia

U2 - 10.1016/j.toxicon.2015.03.001

DO - 10.1016/j.toxicon.2015.03.001

M3 - Journal article

VL - 99

SP - 23

EP - 35

JO - Toxicon

JF - Toxicon

SN - 0041-0101

ER -

ID: 134705042