Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol

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Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol. / Frydenvang, Karla; Verkade-Vreeker, Marlies C A; Dohmen, Floor; Commandeur, Jan N M; Rafiq, Maria; Mirza, Osman; Jørgensen, Flemming Steen; Geerke, Daan P.

In: PLoS ONE, Vol. 14, No. 5, e0217292, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Frydenvang, K, Verkade-Vreeker, MCA, Dohmen, F, Commandeur, JNM, Rafiq, M, Mirza, O, Jørgensen, FS & Geerke, DP 2019, 'Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol', PLoS ONE, vol. 14, no. 5, e0217292. https://doi.org/10.1371/journal.pone.0217292

APA

Frydenvang, K., Verkade-Vreeker, M. C. A., Dohmen, F., Commandeur, J. N. M., Rafiq, M., Mirza, O., ... Geerke, D. P. (2019). Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol. PLoS ONE, 14(5), [e0217292]. https://doi.org/10.1371/journal.pone.0217292

Vancouver

Frydenvang K, Verkade-Vreeker MCA, Dohmen F, Commandeur JNM, Rafiq M, Mirza O et al. Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol. PLoS ONE. 2019;14(5). e0217292. https://doi.org/10.1371/journal.pone.0217292

Author

Frydenvang, Karla ; Verkade-Vreeker, Marlies C A ; Dohmen, Floor ; Commandeur, Jan N M ; Rafiq, Maria ; Mirza, Osman ; Jørgensen, Flemming Steen ; Geerke, Daan P. / Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol. In: PLoS ONE. 2019 ; Vol. 14, No. 5.

Bibtex

@article{83cdd2bdbe514e3f81aa8f74eea124b9,
title = "Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol",
abstract = "The bacterial Cytochrome P450 (CYP) BM3 (CYP102A1) is one of the most active CYP isoforms. BM3 mutants can serve as a model for human drug-metabolizing CYPs and/or as biocatalyst for selective formation of drug metabolites. Hence, molecular and computational biologists have in the last two decades shown strong interest in the discovery and design of novel BM3 variants with optimized activity and selectivity for substrate conversion. This led e.g. to the discovery of mutant M11 that is able to metabolize a variety of drugs and drug-like compounds with relatively high activity. In order to further improve our understanding of CYP binding and reactions, we performed a co-crystallization study of mutant M11 and report here the three-dimensional structure M11 in complex with dithiothreitol (DTT) at a resolution of 2.16 {\AA}. The structure shows that DTT can coordinate to the Fe atom in the heme group. UV/Vis spectroscopy and molecular dynamics simulation studies underline this finding and as first structure of the CYP BM3 mutant M11 in complex with a ligand, it offers a basis for structure-based design of novel mutants.",
author = "Karla Frydenvang and Verkade-Vreeker, {Marlies C A} and Floor Dohmen and Commandeur, {Jan N M} and Maria Rafiq and Osman Mirza and J{\o}rgensen, {Flemming Steen} and Geerke, {Daan P}",
year = "2019",
doi = "10.1371/journal.pone.0217292",
language = "English",
volume = "14",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS

TY - JOUR

T1 - Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol

AU - Frydenvang, Karla

AU - Verkade-Vreeker, Marlies C A

AU - Dohmen, Floor

AU - Commandeur, Jan N M

AU - Rafiq, Maria

AU - Mirza, Osman

AU - Jørgensen, Flemming Steen

AU - Geerke, Daan P

PY - 2019

Y1 - 2019

N2 - The bacterial Cytochrome P450 (CYP) BM3 (CYP102A1) is one of the most active CYP isoforms. BM3 mutants can serve as a model for human drug-metabolizing CYPs and/or as biocatalyst for selective formation of drug metabolites. Hence, molecular and computational biologists have in the last two decades shown strong interest in the discovery and design of novel BM3 variants with optimized activity and selectivity for substrate conversion. This led e.g. to the discovery of mutant M11 that is able to metabolize a variety of drugs and drug-like compounds with relatively high activity. In order to further improve our understanding of CYP binding and reactions, we performed a co-crystallization study of mutant M11 and report here the three-dimensional structure M11 in complex with dithiothreitol (DTT) at a resolution of 2.16 Å. The structure shows that DTT can coordinate to the Fe atom in the heme group. UV/Vis spectroscopy and molecular dynamics simulation studies underline this finding and as first structure of the CYP BM3 mutant M11 in complex with a ligand, it offers a basis for structure-based design of novel mutants.

AB - The bacterial Cytochrome P450 (CYP) BM3 (CYP102A1) is one of the most active CYP isoforms. BM3 mutants can serve as a model for human drug-metabolizing CYPs and/or as biocatalyst for selective formation of drug metabolites. Hence, molecular and computational biologists have in the last two decades shown strong interest in the discovery and design of novel BM3 variants with optimized activity and selectivity for substrate conversion. This led e.g. to the discovery of mutant M11 that is able to metabolize a variety of drugs and drug-like compounds with relatively high activity. In order to further improve our understanding of CYP binding and reactions, we performed a co-crystallization study of mutant M11 and report here the three-dimensional structure M11 in complex with dithiothreitol (DTT) at a resolution of 2.16 Å. The structure shows that DTT can coordinate to the Fe atom in the heme group. UV/Vis spectroscopy and molecular dynamics simulation studies underline this finding and as first structure of the CYP BM3 mutant M11 in complex with a ligand, it offers a basis for structure-based design of novel mutants.

U2 - 10.1371/journal.pone.0217292

DO - 10.1371/journal.pone.0217292

M3 - Journal article

VL - 14

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 5

M1 - e0217292

ER -

ID: 218710654