Structure of the first PDZ domain of human PSD-93

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Monica Fiorentini, Ann Kallehauge Nielsen, Ole Kristensen, Jette Sandholm Kastrup, Michael Gajhede

The crystal structure of the PDZ1 domain of human PSD-93 has been determined to 2.0 A resolution. The PDZ1 domain forms a crystallographic trimer that is also predicted to be stable in solution. The main contributions to the stabilization of the trimer seem to arise from interactions involving the PDZ1-PDZ2 linker region at the extreme C-terminus of PDZ1, implying that the oligomerization that is observed is not of biological significance in full-length PSD-93. Comparison of the structures of the binding cleft of PSD-93 PDZ1 with the previously reported structures of PSD-93 PDZ2 and PDZ3 as well as of the closely related human PSD-95 PDZ1 shows that they are very similar in terms of amino-acid composition. However, the cleft is significantly narrower in PSD-95. This could be part of the basis of peptide selectivity between PSD-93 PDZ1 and PSD-95 PDZ1.
Original languageEnglish
JournalActa Crystallographica. Section F : Structural Biology and Crystallization Communications
Volume65
Issue number12
Pages (from-to)1254-1257
ISSN1744-3091
DOIs
Publication statusPublished - 2009

ID: 17114936