The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines

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The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines. / De Munnik, Sabrina M.; Kooistra, Albert J.; Van Offenbeek, Jody; Nijmeijer, Saskia; De Graaf, Chris; Smit, Martine J.; Leurs, Rob; Vischer, Henry F.

In: PLOS ONE, Vol. 10, No. 4, e0124486, 20.04.2015.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

De Munnik, SM, Kooistra, AJ, Van Offenbeek, J, Nijmeijer, S, De Graaf, C, Smit, MJ, Leurs, R & Vischer, HF 2015, 'The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines', PLOS ONE, vol. 10, no. 4, e0124486. https://doi.org/10.1371/journal.pone.0124486

APA

De Munnik, S. M., Kooistra, A. J., Van Offenbeek, J., Nijmeijer, S., De Graaf, C., Smit, M. J., ... Vischer, H. F. (2015). The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines. PLOS ONE, 10(4), [e0124486]. https://doi.org/10.1371/journal.pone.0124486

Vancouver

De Munnik SM, Kooistra AJ, Van Offenbeek J, Nijmeijer S, De Graaf C, Smit MJ et al. The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines. PLOS ONE. 2015 Apr 20;10(4). e0124486. https://doi.org/10.1371/journal.pone.0124486

Author

De Munnik, Sabrina M. ; Kooistra, Albert J. ; Van Offenbeek, Jody ; Nijmeijer, Saskia ; De Graaf, Chris ; Smit, Martine J. ; Leurs, Rob ; Vischer, Henry F. / The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines. In: PLOS ONE. 2015 ; Vol. 10, No. 4.

Bibtex

@article{8ca83f3b8b7d4c2b95a15251e9a397c3,
title = "The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines",
abstract = "Kaposi's sarcoma-associated herpesvirus-infected cells express the virally encoded G protein-coupled receptor ORF74. Although ORF74 is constitutively active, it binds human CXC chemokines that modulate this basal activity. ORF74-induced signaling has been demonstrated to underlie the development of the angioproliferative tumor Kaposi's sarcoma. Whereas G protein-dependent signaling of ORF74 has been the subject of several studies, the interaction of this viral GPCR with β-arrestins has hitherto not been investigated. Bioluminescence resonance energy transfer experiments demonstrate that ORF74 recruits β-arrestins and subsequently internalizes in response to human CXCL1 and CXCL8, but not CXCL10. Internalized ORF74 traffics via early endosomes to recycling and late endosomes. Site-directed mutagenesis and homology modeling identified four serine and threonine residues at the distal end of the intracellular carboxyl-terminal of ORF74 that are required for β-arrestin recruitment and subsequent endocytic trafficking. Hijacking of the human endocytic trafficking machinery is a previously unrecognized action of ORF74.",
author = "{De Munnik}, {Sabrina M.} and Kooistra, {Albert J.} and {Van Offenbeek}, Jody and Saskia Nijmeijer and {De Graaf}, Chris and Smit, {Martine J.} and Rob Leurs and Vischer, {Henry F.}",
year = "2015",
month = "4",
day = "20",
doi = "10.1371/journal.pone.0124486",
language = "English",
volume = "10",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - The viral G protein-coupled receptor ORF74 hijacks β-arrestins for endocytic trafficking in response to human chemokines

AU - De Munnik, Sabrina M.

AU - Kooistra, Albert J.

AU - Van Offenbeek, Jody

AU - Nijmeijer, Saskia

AU - De Graaf, Chris

AU - Smit, Martine J.

AU - Leurs, Rob

AU - Vischer, Henry F.

PY - 2015/4/20

Y1 - 2015/4/20

N2 - Kaposi's sarcoma-associated herpesvirus-infected cells express the virally encoded G protein-coupled receptor ORF74. Although ORF74 is constitutively active, it binds human CXC chemokines that modulate this basal activity. ORF74-induced signaling has been demonstrated to underlie the development of the angioproliferative tumor Kaposi's sarcoma. Whereas G protein-dependent signaling of ORF74 has been the subject of several studies, the interaction of this viral GPCR with β-arrestins has hitherto not been investigated. Bioluminescence resonance energy transfer experiments demonstrate that ORF74 recruits β-arrestins and subsequently internalizes in response to human CXCL1 and CXCL8, but not CXCL10. Internalized ORF74 traffics via early endosomes to recycling and late endosomes. Site-directed mutagenesis and homology modeling identified four serine and threonine residues at the distal end of the intracellular carboxyl-terminal of ORF74 that are required for β-arrestin recruitment and subsequent endocytic trafficking. Hijacking of the human endocytic trafficking machinery is a previously unrecognized action of ORF74.

AB - Kaposi's sarcoma-associated herpesvirus-infected cells express the virally encoded G protein-coupled receptor ORF74. Although ORF74 is constitutively active, it binds human CXC chemokines that modulate this basal activity. ORF74-induced signaling has been demonstrated to underlie the development of the angioproliferative tumor Kaposi's sarcoma. Whereas G protein-dependent signaling of ORF74 has been the subject of several studies, the interaction of this viral GPCR with β-arrestins has hitherto not been investigated. Bioluminescence resonance energy transfer experiments demonstrate that ORF74 recruits β-arrestins and subsequently internalizes in response to human CXCL1 and CXCL8, but not CXCL10. Internalized ORF74 traffics via early endosomes to recycling and late endosomes. Site-directed mutagenesis and homology modeling identified four serine and threonine residues at the distal end of the intracellular carboxyl-terminal of ORF74 that are required for β-arrestin recruitment and subsequent endocytic trafficking. Hijacking of the human endocytic trafficking machinery is a previously unrecognized action of ORF74.

UR - http://www.scopus.com/inward/record.url?scp=84928905603&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0124486

DO - 10.1371/journal.pone.0124486

M3 - Journal article

VL - 10

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 4

M1 - e0124486

ER -

ID: 199375904