Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

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Walther Parson, Harald Niederstätter, Alexandra Lindinger, Peter Gill, Niels Morling

The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.
Original languageEnglish
JournalForensic Science International: Genetics
Volume2
Issue number3
Pages (from-to)238-42
Number of pages4
ISSN1872-4973
DOIs
Publication statusPublished - 2008

Bibliographical note

Keywords: Bacteriophage lambda; Chromosomes, Human, Y; Complex Mixtures; DNA; DNA Fingerprinting; DNA, Viral; Forensic Genetics; Genotype; Humans; Male; Microsatellite Repeats; Reference Standards; Sensitivity and Specificity; Specimen Handling

ID: 13059814