In Vivo Neutralization of Myotoxin II, a Phospholipase A2Homologue from Bothrops asper Venom, Using Peptides Discovered via Phage Display Technology

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  • Andreas H. Laustsen
  • Bengt H. Gless
  • Timothy P. Jenkins
  • Maria Meyhoff-Madsen
  • Johanna Bjärtun
  • Andreas S. Munk
  • Saioa Oscoz
  • Julián Fernández
  • José María Gutiérrez
  • Bruno Lomonte
  • Brian Lohse

Many snake venom toxins cause local tissue damage in prey and victims, which constitutes an important pathology that is challenging to treat with existing antivenoms. One of the notorious toxins that causes such effects is myotoxin II present in the venom of the Central and Northern South American viper, Bothrops asper. This Lys49 PLA2 homologue is devoid of enzymatic activity and causes myotoxicity by disrupting the cell membranes of muscle tissue. To improve envenoming therapy, novel approaches are needed, warranting the discovery and development of inhibitors that target key toxins that are currently difficult to neutralize. Here, we report the identification of a new peptide (JB006), discovered using phage display technology, that is capable of binding to and neutralizing the toxic effects of myotoxin II in vitro and in vivo. Through computational modeling, we further identify hypothetical binding interactions between the toxin and the peptide to enable further development of inhibitors that can neutralize myotoxin II.

Original languageEnglish
JournalACS Omega
Volume7
Issue number18
Pages (from-to)15561–15569
ISSN2470-1343
DOIs
Publication statusPublished - 2022

Bibliographical note

Funding Information:
This work was supported by the Department of Drug Design and Pharmacology, University of Copenhagen, and the Tech Transfer Office, University of Copenhagen (to B.L.). We also thank Professor Christian Adam Olsen from the University of Copenhagen for access to peptide synthesis facilities, Lorenzo Seneci from the Technical University of Denmark for help with formatting the text and figures, and Christoffer V. Sørensen from the Technical University of Denmark for help with proofreading.

ID: 308039046