Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

Research output: Contribution to journalJournal articlepeer-review

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Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. / Khoo, K. K.; Galleano, I.; Gasparri, F.; Wieneke, R.; Harms, H.; Poulsen, M. H.; Chua, H. C.; Wulf, M.; Tampé, R.; Pless, S. A.

In: Nature Communications, Vol. 11, No. 1, 2284, 01.12.2020.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Khoo, KK, Galleano, I, Gasparri, F, Wieneke, R, Harms, H, Poulsen, MH, Chua, HC, Wulf, M, Tampé, R & Pless, SA 2020, 'Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing', Nature Communications, vol. 11, no. 1, 2284. https://doi.org/10.1038/s41467-020-16208-6

APA

Khoo, K. K., Galleano, I., Gasparri, F., Wieneke, R., Harms, H., Poulsen, M. H., Chua, H. C., Wulf, M., Tampé, R., & Pless, S. A. (2020). Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. Nature Communications, 11(1), [2284]. https://doi.org/10.1038/s41467-020-16208-6

Vancouver

Khoo KK, Galleano I, Gasparri F, Wieneke R, Harms H, Poulsen MH et al. Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. Nature Communications. 2020 Dec 1;11(1). 2284. https://doi.org/10.1038/s41467-020-16208-6

Author

Khoo, K. K. ; Galleano, I. ; Gasparri, F. ; Wieneke, R. ; Harms, H. ; Poulsen, M. H. ; Chua, H. C. ; Wulf, M. ; Tampé, R. ; Pless, S. A. / Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. In: Nature Communications. 2020 ; Vol. 11, No. 1.

Bibtex

@article{1a90272c88d946bba5d5501a0a89b87f,
title = "Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing",
abstract = "Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.",
author = "Khoo, {K. K.} and I. Galleano and F. Gasparri and R. Wieneke and H. Harms and Poulsen, {M. H.} and Chua, {H. C.} and M. Wulf and R. Tamp{\'e} and Pless, {S. A.}",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41467-020-16208-6",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

AU - Khoo, K. K.

AU - Galleano, I.

AU - Gasparri, F.

AU - Wieneke, R.

AU - Harms, H.

AU - Poulsen, M. H.

AU - Chua, H. C.

AU - Wulf, M.

AU - Tampé, R.

AU - Pless, S. A.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.

AB - Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.

U2 - 10.1038/s41467-020-16208-6

DO - 10.1038/s41467-020-16208-6

M3 - Journal article

C2 - 32385250

AN - SCOPUS:85084720502

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 2284

ER -

ID: 245323680