Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1
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Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.
Original language | English |
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Journal | Acta Crystallographica. Section F : Structural Biology and Crystallization Communications |
Volume | 61 |
Issue number | Pt 9 |
Pages (from-to) | 858-60 |
Number of pages | 2 |
ISSN | 1744-3091 |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Animals; Crystallization; Crystallography, X-Ray; Fibronectins; Gene Expression; Immunoglobulins; Mice; Neural Cell Adhesion Molecule L1
ID: 21605053