Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol
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Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol. / Frydenvang, Karla; Verkade-Vreeker, Marlies C A; Dohmen, Floor; Commandeur, Jan N M; Rafiq, Maria; Mirza, Osman; Jørgensen, Flemming Steen; Geerke, Daan P.
In: PLoS ONE, Vol. 14, No. 5, e0217292, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol
AU - Frydenvang, Karla
AU - Verkade-Vreeker, Marlies C A
AU - Dohmen, Floor
AU - Commandeur, Jan N M
AU - Rafiq, Maria
AU - Mirza, Osman
AU - Jørgensen, Flemming Steen
AU - Geerke, Daan P
PY - 2019
Y1 - 2019
N2 - The bacterial Cytochrome P450 (CYP) BM3 (CYP102A1) is one of the most active CYP isoforms. BM3 mutants can serve as a model for human drug-metabolizing CYPs and/or as biocatalyst for selective formation of drug metabolites. Hence, molecular and computational biologists have in the last two decades shown strong interest in the discovery and design of novel BM3 variants with optimized activity and selectivity for substrate conversion. This led e.g. to the discovery of mutant M11 that is able to metabolize a variety of drugs and drug-like compounds with relatively high activity. In order to further improve our understanding of CYP binding and reactions, we performed a co-crystallization study of mutant M11 and report here the three-dimensional structure M11 in complex with dithiothreitol (DTT) at a resolution of 2.16 Å. The structure shows that DTT can coordinate to the Fe atom in the heme group. UV/Vis spectroscopy and molecular dynamics simulation studies underline this finding and as first structure of the CYP BM3 mutant M11 in complex with a ligand, it offers a basis for structure-based design of novel mutants.
AB - The bacterial Cytochrome P450 (CYP) BM3 (CYP102A1) is one of the most active CYP isoforms. BM3 mutants can serve as a model for human drug-metabolizing CYPs and/or as biocatalyst for selective formation of drug metabolites. Hence, molecular and computational biologists have in the last two decades shown strong interest in the discovery and design of novel BM3 variants with optimized activity and selectivity for substrate conversion. This led e.g. to the discovery of mutant M11 that is able to metabolize a variety of drugs and drug-like compounds with relatively high activity. In order to further improve our understanding of CYP binding and reactions, we performed a co-crystallization study of mutant M11 and report here the three-dimensional structure M11 in complex with dithiothreitol (DTT) at a resolution of 2.16 Å. The structure shows that DTT can coordinate to the Fe atom in the heme group. UV/Vis spectroscopy and molecular dynamics simulation studies underline this finding and as first structure of the CYP BM3 mutant M11 in complex with a ligand, it offers a basis for structure-based design of novel mutants.
U2 - 10.1371/journal.pone.0217292
DO - 10.1371/journal.pone.0217292
M3 - Journal article
C2 - 31125381
VL - 14
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 5
M1 - e0217292
ER -
ID: 218710654