Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids
Research output: Contribution to journal › Journal article › Research › peer-review
When highly unsaturated fatty acids are added to cell cultures, it can become important to include antioxidants in the culture medium to prevent cytotoxic peroxidation. To find an optimal antioxidant for this purpose, the effect of 50 µM a-tocopherol, ¿-tocopherol, a-tocopheryl acetate, a- tocopheryl acid succinate, or a-tocopheryl phosphate, or of 1 µM N,N'- diphenyl-1,4-phenylenediamine, was investigated with respect to the agent's ability to prevent lactate dehydrogenase leakage in long-term rat hepatocyte cultures supplemented with 0.5 mM highly unsaturated fatty acids. Formation of thiobarbituric acid reactive substances in the cultures was also measured. a-Tocopheryl acid succinate was found to be the most effective cytoprotective compound, followed by N,N'-diphenyl-1,4-phenylenediamine, a- tocopherol, ¿-tocopherol and a-tocopheryl acetate, and a-tocopheryl phosphate was without effect.
Original language | English |
---|---|
Journal | Lipids |
Volume | 29 |
Issue number | 5 |
Pages (from-to) | 369-372 |
Number of pages | 4 |
ISSN | 0024-4201 |
Publication status | Published - 1994 |
ID: 262264