TY - JOUR

T1 - Evaluation of Ethno-Veterinary Grasses from Suburbs through Microscopic Technique

T2 - Insights into Antioxidant Defense System and Phenol

AU - Raza, Awais

AU - Hanif, Uzma

AU - Ali, Shanza

AU - Ali, Baber

AU - Khan, Muhammad Nauman

AU - Fatima, Mahreen

AU - Rafique, Sadia

AU - Rashid, Muhammad

AU - Sher, Malik Muhammad

AU - Ullah, Riaz

N1 - Publisher Copyright: © 2024, HARD Publishing Company. All rights reserved.

PY - 2024

Y1 - 2024

N2 - This research work was conducted to investigate the anatomical and antioxidant studies of some ethnoveterinary grasses (Cenchrus pennisetiformis and Dichanthium annulatum). The anatomical characteristics (compact epidermal layer, large cortical cells, thickened sclerenchyma, central and scattered vascular bundles, large metaxylem, small protoxylem, and centrally located pith) were observed. For the antioxidant activity studies, the crude methanol extract was prepared by maceration techniques and different fractions were prepared (n-hexane, chloroform, and aqueous solution). The maximum antioxidant potential in DPPH was shown by C. pennisetiformis at a concentration of 250 µL in the chloroform fraction, while D. annulatum showed a maximum value of 68.47±0.30 at a concentration of 250 µL in the methanolic extract. In both species, chloroform extract in TAA showed maximum potency at a concentration of 500 µL, but C. pennisetiformis showed a higher value (i.e. 1.15±0.0018) and D. annulatum showed a lever value (i.e. 1.05±0.0017). The best reducing power in FRAP was shown by D. annulatum followed by C. pennisetiformis. C. pennisetiformis showed the highest value in the chloroform fraction while D. annulatum showed a higher significant value in the methanol fraction. These grasses have the potential to control several diseases caused by ROS in animals.

AB - This research work was conducted to investigate the anatomical and antioxidant studies of some ethnoveterinary grasses (Cenchrus pennisetiformis and Dichanthium annulatum). The anatomical characteristics (compact epidermal layer, large cortical cells, thickened sclerenchyma, central and scattered vascular bundles, large metaxylem, small protoxylem, and centrally located pith) were observed. For the antioxidant activity studies, the crude methanol extract was prepared by maceration techniques and different fractions were prepared (n-hexane, chloroform, and aqueous solution). The maximum antioxidant potential in DPPH was shown by C. pennisetiformis at a concentration of 250 µL in the chloroform fraction, while D. annulatum showed a maximum value of 68.47±0.30 at a concentration of 250 µL in the methanolic extract. In both species, chloroform extract in TAA showed maximum potency at a concentration of 500 µL, but C. pennisetiformis showed a higher value (i.e. 1.15±0.0018) and D. annulatum showed a lever value (i.e. 1.05±0.0017). The best reducing power in FRAP was shown by D. annulatum followed by C. pennisetiformis. C. pennisetiformis showed the highest value in the chloroform fraction while D. annulatum showed a higher significant value in the methanol fraction. These grasses have the potential to control several diseases caused by ROS in animals.

KW - anatomical studies

KW - animals

KW - antioxidants

KW - ethnobotany

KW - grasses

U2 - 10.15244/pjoes/176049

DO - 10.15244/pjoes/176049

M3 - Journal article

AN - SCOPUS:85189986024

VL - 33

SP - 3367

EP - 3379

JO - Polish Journal of Environmental Studies

JF - Polish Journal of Environmental Studies

SN - 1230-1485

IS - 3

ER -