Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra
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Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra. / Thomsen, S.; Hansen, Harald S.; Nyman, U.
In: Planta Medica, Vol. 57, No. 3, 01.01.1991, p. 232-236.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra
AU - Thomsen, S.
AU - Hansen, Harald S.
AU - Nyman, U.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Phytolacca dodecandra (L'Herit) grown in cell cultures was investigated for content of ribosome-inhibiting proteins, which was evaluated hy measuring inhibition of protein synthesis in a cell-free rat liver extract. Calli initiated from leaf, cotyledon, radicle, and hypocotyl and suspension cells initiated from leaf and cotyledon exhibited protein synthesis-inhibiting activity. Ribosome-inhibiting proteins were purified at least 14 times from suspension cells of P. dodecandra. The purified protein fraction contained two proteins as seen by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The relative molecular masses were 30,000 and 31,000 and they showed a pI > 9.3. These new RIP's were shown to be different from dodecandrin with respect to molecular mass.
AB - Phytolacca dodecandra (L'Herit) grown in cell cultures was investigated for content of ribosome-inhibiting proteins, which was evaluated hy measuring inhibition of protein synthesis in a cell-free rat liver extract. Calli initiated from leaf, cotyledon, radicle, and hypocotyl and suspension cells initiated from leaf and cotyledon exhibited protein synthesis-inhibiting activity. Ribosome-inhibiting proteins were purified at least 14 times from suspension cells of P. dodecandra. The purified protein fraction contained two proteins as seen by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The relative molecular masses were 30,000 and 31,000 and they showed a pI > 9.3. These new RIP's were shown to be different from dodecandrin with respect to molecular mass.
UR - http://www.scopus.com/inward/record.url?scp=0025909306&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:0025909306
VL - 57
SP - 232
EP - 236
JO - Planta Medica
JF - Planta Medica
SN - 0032-0943
IS - 3
ER -
ID: 45561672