CPHSAXS, Facility for biological SAXS
Picture: overview of CPHSAXS
The Facility has focus on BioSAXS and is available for research as well as screening and optimization of experiments before commencement of experiments at large scale facilities. The facility is funded by the Novo Nordisk foundation.
Small angle X-ray scattering is a solution technique which for ideal systems can provide information about the size, shape and molecular weight of a sample. The facility is dedicated to biological samples, such as proteins, lipids and colloidal systems in solution.
The BioXolver L is a state of the art small angle X-ray scattering instrument with the ability to probe soft matter systems up to 150 nm.
The BioXolver L provides 2*96 well plates for rapid screening of samples with temperature control.
Sample amount: The lowest amount possible is 5 µL in the flow cell. The concentration depends on the size of the sample but often a concentration range of 1-10 mg/mL is suitable.
Sample preparation: matching buffer is important. Best prepared by dialysis! Buffer should be available in sufficient quantity for dilution and buffer measurements, both before and after sample.
Sample stability: Is the sample monodisperse, how long is the sample stable at room temperature, does it need to be prepared on site, etc.
Temperature (instrument capability: 4-60°C)
q-range (Possible from 0.003-2.8Å-1)
Currently there is no cost for academic users for experiments performed at CPHSAXS
Please contact the facility manager or for specific sample information, a member of the steering committee for further information.
Developing Inhibitors of the p47phox-p22phox Protein-Protein Interaction by Fragment-Based Drug Discovery. S. M.Ø. Solbak, J. Zang, D. Narayanan, L. J. Høj, S. Bucciarelli, C. Softley, S. Meier, A. E. Langkilde, C. H. Gotfredsen, M. Sattler, A. Bach.
Journal of Medicinal Chemistry,2020, 63, 3, 1156-1177.
The structural basis of fungal glucuronoyl esterase activity on natural substrates. H. A Ernst, C. Mosbech, A. E. Langkilde, P. Westh, A. S. Meyer, J. A. Wittrup, S. Larsen.
Nature Communications, 2020, 11, 1026, 1-12.
Oxidation of Polyunsaturated Lipid Membranes by UV-Activated Titanium Dioxide Nanoparticles: Role of pH and Salinity. E. Parra-Ortiz, S. Malekkhaiat-Häffner, T. Saerbeck, M. Skoda, K. Browning, and M. Malmsten
ACS Appl. Mater. Interf. 2020, 12, 32446.
Composition Effects on Photooxidative Membrane Destabilization by TiO2Nanoparticles. S. Malekkhaiat-Häffner, E. Parra-Ortiz, M. Skoda, T. Saerbeck, K.L. Browning, and M. Malmsten,
J. Colloid Interface Sci. 2021, 584, 19.
Disentangling the role of solvent polarity and protein solvation in folding and self-assembly of α-lactalbumin.
S. Bucciarelli, E. S. Sayedi, S. Osella, B. Trzaskowski, K. J. Vissing, B. Vestergaard, V. Foderà
J. Colloid Interface Sci. 2020, 749-761
Molecular architecture of the Jumonji C family histone demethylase KDM5B
J. Dorosz, L. H. Kristensen, N. G. Aduri, O. Mirza, R. Lousen, S. Bucciarelli, V. Mehta, S. Sellés-Baiget, S. M. Ø. Solbak, A. Bach, P. Mesa, P. A. Hernandez, G. Montoya, T. T. T. N. Nguyen, K. D. Rand, T. Boesen, M. Gajhede
Sci Rep 9, 2019, 4019
a. When publishing research performed at CPHSAXS please acknowledge us as following:
We acknowledge the University of Copenhagen, Small angle X-ray facility, CPHSAXS, funded by the Novo Nordisk Foundation (grant no. NNF19OC0055857). https://drug.ku.dk/core-facilities/cphsaxs/
b. In the methods description we suggest:
Small-angle X-ray scattering (SAXS) studies were performed at the CPHSAXS Facility, University of Copenhagen, on a Xenocs© BioXolver L with a wavelength of λ = 1.34 Å. Primary data reduction was made in BIOXTAS RAW.
See publication guidelines: https://doi.org/10.1107/S0907444912012073 and https://doi.org/10.1107/S2059798317011597
CPHSAXS is founded by the Novo Nordisk foundation.